Adenosine Deaminase (ADA)Assay Kit – Bulk Reagents

US$249.00

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Selling unit: per 100ml

Product Name

Common name: Adenosine deaminase (ADA) assay kit (oxidase rate method)

English name: ADA Reagent Kit (PODKinetic Method)

Reagent Ratio

2:1,3:1,4:1 can be used.

Intended Use

This kit is used to determine the ADA activity in human serum or plasma.

Elevated ADA activity in serum is seen in acute hepatitis, alcoholic liver fibrosis, chronic active hepatitis, cirrhosis, viral hepatitis and hepatocellular carcinoma, as well as in tuberculosis exudate. The ADA activity is also of high clinical value in the differential diagnosis of tuberculous meningitis.

Test Principle

ADA

Adenosine + H2 0 ──→ Hypoxanthine nucleoside + NH3

PNP

Hypoxanthine riboside + P ──→ Hypoxanthine + ribulose-1-phosphate

XOD

Hypoxanthine + 2H2 0 + 2O2 ──→ 2H2 02 + uric acid

POD

2H2 02 + 4-AAP+TOOS ──→ 4H2 0+colored quinone

Main Components

Reagent components included in the product.

Adenosine Deaminase (ADA) Assay Kit

Adenosine Deaminase (ADA) Assay Kit

 

*Components are not interchangeable in kits with different lot numbers.

*Reagent components not included in the product, but necessary for the test: outsourced normal and abnormal QCs and calibrators.

Storage Conditions And Expiration Date

The kit is stored at 2-8°C away from light and is valid for one year.

Reagents that have been opened are careful not to be contaminated, and reagents are stable in the instrument compartment (2-8°C) for one month.

The reagents must not be frozen.

Applicable Instruments

Hitachi 7180/7600; Olympus AU680/2700; Toshiba TBA120; Myriad BS2000M/480; Siemens ADVIA 1800/2400 series automatic biochemical analyzers.

Sample Request

Fresh serum or plasma (EDTA anticoagulation or heparin anticoagulation). The ADA in the sample is stable for 7 days when stored at 2-8°C. If the specimen cannot be measured in time, it should be stored at -20°C and can be stable for 30 days, avoid repeated freezing and thawing.

Test Method

  1. Reagent preparation: liquid reagents are ready to use out of the bottle.
  2. test conditions: (different test instruments can be requested according to the different parameters on the machine)

Adenosine Deaminase (ADA)

Operation steps.

Adenosine Deaminase (ADA)

Reagents and sample volumes can be increased or decreased in proportion to the requirements of different biochemical analyzers.

  1. Calibration procedure.

The use of matching calibrators is recommended.

  1. QC control procedures.

It is recommended that each laboratory establish its own quality control system and select appropriate quality control products for quality control. The measured values of QC products should be within the specified range. If outside the specified range, it is necessary to take appropriate measures or contact the manufacturer.

  1. Calculation.

ADA activity (U/L) = (ΔA样品 /min-ΔA空白 /min) × C标准 / (ΔA标准 /min-ΔA空白 /min)

Positive Judgment Value Or Reference Interval

0~25 U/L

Each laboratory should establish its own normal reference range according to the region and population.

Interpretation Of Test Results

The determination of ADA in serum or plasma is only one of the indicators used by the clinician to diagnose the patient. The clinician must also make a comprehensive judgment based on the patient’s physical symptoms, medical history, and other diagnostic items and diagnostic tools.

Limitations Of The Test Method

Ascorbic acid ≤ 5 mg/dL, triglycerides ≤ 500 mg/dL and hemoglobin ≤ 500 mg/dL did not interfere with the determination.

Product Performance Index

Absorbance of reagent blank: wavelength 548 nm, optical diameter 1.0 cm, temperature 37°C, A0  ≤ 0.2.

Absorbance change rate of reagent blank: △A/min ≤ 0.001 at wavelength 548 nm and optical diameter 1.0 cm.

Analytical sensitivity: The kit tests 10 U/L of the test substance with absorbance change rate ΔA/min ≥ 0.004.

Linearity range: test serum samples, reagent linearity in the interval of 1~200 U/L, linear correlation coefficient|r| should not be less than 0.975; in the interval of [1~20] U/L, the absolute deviation of linearity should not exceed 2 U/L; in the interval of (20~200] U/L, the relative deviation of linearity should not exceed ±10%.

Precision: repeatability CV ≤ 5%; relative extreme difference between batches ≤ 6 %.

Accuracy: Relative deviation ≤ 10%.

Caution

  1. This product is for in vitro diagnosis only.
  2. Avoid contamination when using the reagent, the container used must be clean, and please take necessary precautions, do not swallow, and avoid contact with skin and mucous membrane.
  3. Please dispose of the measured samples and waste liquids in accordance with the relevant national and local laws and regulations.
  4. Do not mix R1 and R2 into a single reagent.
  5. When changing the reagent lot number, please recalibrate.

Reference

  1. Kobayashi F, Ikeda T, Marumo F, Sato C: Adenosine deaminase isoenzymes in liver disease. Am. J. Gastroenterol. 88: 266-271 (1993)
  2. Oswaldo Cruz94 (3) 383-386 (1999).
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