Aspartate Aminotransferase (AST) Assay Kit – Bulk Reagents


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Selling unit: per 100ml

Product Name

Common name: Aspartate Aminotransferase (AST ) assay kit (IFCC rate method)

English Name.AST Reagent Kit (IFCC Kinetic Method)

Reagent Ratio

3:1, 4:1, 5:1, common ratio 3:1, other ratios need to be customized

Intended Use

Used to determine the viability of AST in human serum or plasma.

Myocardial infarction, viral hepatitis, hepatic necrosis, cirrhosis, and myotonic dystrophy all cause elevated aspartate aminotransferase activity in serum or plasma.

Test Principle

The AST in the sample catalyzes the formation of oxaloacetate and L-glutamic acid from L-aspartate and α-ketoglutarate in the reagent. Oxaloacetate is then catalyzed with NADH in the reagent by malate dehydrogenase (MDH) to form L-malate, and NADH is oxygenated to NAD+ , which causes a decrease in absorbance at 340 nm. By monitoring the rate of decrease in absorbance at 340 nm, the activity of AST in the sample can be calculated.

Main components

  1. Reagent components contained in the product.

Aspartate Aminotransferase (AST ) assay kit

*The components of the kits are not interchangeable between batches.

*Reagent components not included in the product, but necessary for the test: outsourced normal and abnormal QCs and calibrators.

Storage Conditions And Expiration Date

The kit is stored at 2-8°C away from light and is valid for one year.

Reagents that have been opened are taken care not to be contaminated and the reagents are stabilized in the instrument compartment (2-8°C) for one month.

The reagents must not be frozen.

Applicable Instruments

Hitachi 7180/7600; Olympus AU680/2700; Toshiba TBA120; Myriad BS2000M/480; Siemens ADVIA 1800/2400 series automatic biochemical analyzers.

Sample Request

The most appropriate sample is fresh, non-haemolyzed serum or plasma. Plasma should be anticoagulated with heparin or EDTA; do not use citrate, oxalate or fluoride. AST viability in samples continues to decrease mildly at room temperature (20-25°C), is stable for 7 days at 2-8°C, and is stable for one month when stored at -20°C.

Test Method

  1. Reagent preparation: liquid reagents are ready to use out of the bottle.
  2. test conditions: (different test instruments can be requested according to the different parameters on the machine)

Aspartate Aminotransferase (AST ) assay kit

Operation steps.

Aspartate Aminotransferase (AST ) assay kit

*Reagents and sample volumes can be increased or decreased in proportion to the requirements of different biochemical analyzers.

  1. Calibration procedure.

Randox calibration sera are recommended. the method of choice for Randox calibration sera is the IFCC/SFBC method, Tris buffer without pyridoxal phosphate, 37°C.

  1. QC control procedures.

It is recommended that each laboratory establish its own quality control system and select appropriate quality control products for quality control. The measured values of QC products should be within the specified range. If outside the specified range, it is necessary to take appropriate measures or contact the manufacturer.

  1. Calculation.

Sample concentration = (sample ΔA /min) / (standard ΔA /min) × standard concentration

Positive Judgment Value Or Reference Interval.


It is recommended that each laboratory establish its own reference range of normal values.


Interpretation Of Test Results

The measurement of AST in serum or plasma is only one of the indicators used by the clinician to make a diagnosis of the patient. The clinician must also make a comprehensive judgment based on the patient’s physical symptoms, medical history, and other diagnostic items and diagnostic tools.

Limitations Of The Test Method

Ascorbic acid ≤ 30 mg/dL and bilirubin ≤ 40 mg/dL did not interfere with the determination.

Product Performance Index

Absorbance of reagent blank: wavelength 340 nm, optical diameter 1.0 cm, temperature 37°C, A0  ≥ 1.0.

Absorbance change rate of reagent blank: △A/min ≤ 0.004 at wavelength 340 nm and optical diameter 1.0 cm.

Analytical sensitivity: The kit tests 130 U/L of the test substance with absorbance change rate ΔA/min ≥ 0.02.

Linearity range: test serum samples, reagent linearity in the interval of 5 to 1200 U/L, linear correlation coefficient |r| should not be less than 0.990; in the interval of 5 to 100 U/L, the absolute deviation of linearity should not exceed 15 U/L; in the interval of (100 to 1200 U/L, the relative deviation of linearity should not exceed ±10%.

Precision: Repeatability CV ≤ 5%; relative extreme difference between batches ≤ 10%.

Accuracy: Relative deviation ≤ 10%.


  1. 1. This product is for in vitro diagnosis only.
  2. 2. Avoid contamination when using the reagent, the container used must be clean, and please take necessary precautions, do not swallow, and avoid contact with skin and mucous membrane.
  3. 3. Please dispose of the measured samples and waste liquids in accordance with the relevant national and local laws and regulations.
  4. 4. Do not mix R1 and R2 into a single reagent.
  5. 5. When changing the reagent lot number, please recalibrate.


  1. Clin. Chem. Acta 105 (1980) S. 147-172.
  2. Thefeld W. et al, Dt. Med. Wschr. 99 (1974), 343.
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