Selling unit: per 100ml
Common name: Mitochondrial Aspartate aminotransferase(mAST) assay kit
English name: Mitochondrial Aspartate aminotransferase Test Kit (mAST)
R1: 5×40 ml R2: 1×50 ml; R1: 3×40 ml R2: 2×15 ml
R1: 4×50 ml R2: 1×50 ml; R1: 8×20 ml R2: 8×5 ml
R1: 1×20 ml R2: 1×5 ml; R1: 1×4 L R2: 1×1 L
In vitro determination of Mitochondrial Aspartate aminotransferase(mAST) in human serum
Aspartate aminotransferase (AST) is widely present in many organs, including heart, liver, skeletal muscle, and kidney in order of abundance. two isozymes of AST, controlled by different genes, are present in the cytoplasm (cAST) and mitochondria (mAST). mAST is an isoenzyme of aspartate aminotransferase located in the mitochondria, and the release of the mitochondrial enzyme is higher in severe tissue injury. This can lead to an increase in serum mAST. An increase in serum mAST reflects the severity of subcellular structural damage and is a sensitive indicator for the diagnosis of hepatocellular damage and myocardial infarction. In liver disease, mAST can be used to evaluate hepatocyte necrosis and determine healing, and to diagnose patients with active alcoholic liver disease; in heart disease, it can determine the effectiveness of treatment and healing of myocardial infarction.
This method is based on the determination of mAST activity in the presence of a protease that completely inhibits the activity of cAST without affecting the activity of mAST, and subsequently using a method for measuring total AST activity.
In the AST rate assay the enzyme coupling reaction equation is
L-aspartic acid + α-ketoglutaric acid Oxaloacetate + L-glutamic acid
Oxaloacetic acid + NADH + H+ L-malic acid + NAD+ + H2 O
Storage Conditions And Expiration Date
The reagents are stored at 2 – 8℃ sealed and protected from light, and are valid for 12 months; the opened reagents are stored at 2 – 8℃ taking care to avoid contamination, and are valid for 30 days.
- fresh serum to avoid hemolysis
- Sample stability: 2~8℃ can be stable for one week.
Open-ended fully automated biochemical analyzer.
- Reagent preparation: liquid reagents can be used when opened
- Measurement conditions
Main wavelength 340nm
Colorimetric cup light diameter 1cm
Type of analysis Rate method
- Calibration procedures
Two-point calibration with deionized water and mAST calibrator. Calibrators are available separately. RANDOX or other traceable calibrators are recommended.
- quality control procedures
The mAST quality control was measured and the test results were within the quality control range before the samples were tested.
- Operation steps
- Calculation method
Sample △A/min Sample concentration = ——- × standard concentration
No less than 100 normal population blood samples were selected through clinical trials and analyzed by fully automated biochemical analysis
The measured values were statistically processed and the reference range was calculated.
It is recommended that each laboratory establish its own reference range!
Limitations of test results.
The Mitochondrial Aspartate aminotransferase(mAST) (mAST) assay is only one of the most important tests that clinicians can perform on patients with
The clinician also has to make a diagnosis based on the patient’s physical symptoms, medical history, and his or her
Its diagnostic items, diagnostic tools for comprehensive judgment.
Interpretation of test results
Human error, sample handling, deviation of analytical instruments, etc. can affect the determination results; when
If individual samples deviate too far from the expected value, they need to be re-measured.
- 1. reagent blank absorbance ≥ 0.800 at 37℃, 340nm wavelength, 1cm optical diameter.
The rate of change of absorbance of the reagent blank when the reagent is added to the test with saline as the sample
(ΔA/min) should be ≤ 0.020.
- Precision: Repeatability CV ≤ 5%; relative extreme difference between batches R ≤ 10%.
- Accuracy: Relative deviation ≤ 10%.
- Linearity range: 0~300U/L, r≥0.990.
- Analytical sensitivity: Mitochondrial Aspartate aminotransferase(mAST)is tested with this kit
The absorbance change rate caused by 1 unit concentration is not less than 0.75×10-4 ΔA/min.
- Stability: The reagent is stored in a light-proof environment without corrosive gases for 12 hours at 2℃～8℃.
- hemoglobin ≤ 500 mg/dL, triglycerides ≤ 1250 mg/dL, ascorbic acid in the sample
No significant interference was observed at ≤60 mg/dL.
- Do not mix or exchange reagents with different lot numbers.
- Reagents should be stored airtight according to the specified method after opening.
- test tubes and other instruments that have come into contact with the test sample should be disposed of in accordance with medical waste disposal methods
- Reagents of different batches cannot be mixed, please re-calibrate when changing reagent batches!
Modern Clinical Biochemical Testing, Xiuming Zhang and Jianzhai Li, 2001, P166