Phospholipid (PLIP) Assay Kit – Bulk Reagents


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Selling unit: per 100ml

Product Name

  1. Common name: Phospholipid (PLIP) assay kit (oxidase method)
  2. English name: Phospholipid (PLIP) Reagent Kit (Oxidase )

Reagent Ratio

3:1, 4:1, 5:1, common ratio 4:1, other ratios need to be customized

Intended Use

This reagent is used to detect the level of PLIP in human serum or plasma.

Increased serum PLIP is seen in diabetes, nephrotic syndrome, chronic hemorrhagic anemia, cirrhosis, hepatic necrosis, biliary obstruction, hypothyroidism, essential hypertension, and syphilis; decreased is seen in hyperthyroidism, acute infectious fever, and dystrophic cirrhosis.

Test Principle

PLIP is hydrolyzed by phospholipase D to produce choline and phosphatidic acid, and choline is oxidized by choline oxidase to produce hydrogen peroxide, which reacts with 4-aminoantipyrine and DAOS to produce a blue dye. This dye has a maximum absorption peak at 600 nm, and the absorption intensity is proportional to the PLIP content in serum.

Main Components

Reagent components included in the product.

Phospholipid (PLIP) assay kit

Phospholipid (PLIP) assay kit

*Components are not interchangeable in kits with different lot numbers.

*Reagent components not included in the product, but necessary for the test: outsourced normal and abnormal QCs and calibrators.

Storage Conditions And Expiration Date

The kit is stored at 2-8°C away from light and is valid for one year.

Reagents that have been opened are careful not to be contaminated, and reagents are stable in the instrument compartment (2-8°C) for one month.

The reagents must not be frozen.

Applicable Instruments

Hitachi 7180/7600; Olympus AU680/2700; Toshiba TBA120; Myriad BS2000M/480; Siemens ADVIA 1800/2400 series automatic biochemical analyzers.

Sample Request

Fresh serum or heparin anticoagulated plasma.

Test Method

  1. Reagent preparation: liquid reagents are ready to use out of the bottle.
  2. test conditions: (different parameters on the machine can be requested according to different testing instruments)

Phospholipid (PLIP) assay kit

Operation steps.

Phospholipid (PLIP) assay kit

Reagents and sample volumes can be increased or decreased in proportion to the requirements of different biochemical analyzers.

  1. Calibration procedure.

The use of a matching calibration serum is recommended.

  1. QC control procedures.

It is recommended that each laboratory establish its own quality control system and select appropriate quality control products for quality control. The measured values of QC products should be within the specified range. If outside the specified range, it is necessary to take appropriate measures or contact the manufacturer.

  1. Calculation.

Content (mg/dL) =(ΔAsample – ΔAblank ) × Cstandard / (ΔAstandard – ΔAblank )

Positive Judgment Value Or Reference Interval

148~250 mg/dL

It is recommended that each laboratory establish its own reference range of normal values.

Interpretation Of Test Results

Ascorbic acid ≤ 30 mg/dL, bilirubin ≤ 40 mg/dL, hemoglobin ≤ 400 mg/dL, celiac disease ≤ 500 mg/dL did not interfere with the determination.

Limitations Of The Test Method

The measurement of PLIP in human serum or plasma is only one of the indicators used by the clinician to make a diagnosis of the patient. The clinician must also make a comprehensive judgment based on the patient’s physical symptoms, medical history, and other diagnostic items and diagnostic tools.

Product Performance Index

Absorbance of reagent blank: wavelength 604 nm, optical diameter 1.0 cm, temperature 37°C, A0  ≤ 0.3.

Analytical sensitivity: the kit tests 100 mg/dL of the test substance, the absorbance change value ΔA ≥ 0.05.

Linearity range: test serum samples, reagent linearity in the interval of 5 to 1000 mg/dL, linear correlation coefficient|r| should not be less than 0.990; in the interval of 5 to 200 mg/dL, the absolute deviation of linearity should not exceed 20 mg/dL; in the interval of (200 to 1000 mg/dL, the relative deviation of linearity should not exceed ±10%.

Precision: repeatability CV ≤ 6%; relative extreme difference between batches ≤ 10%.

Accuracy: Relative deviation ≤ 10%.


  1. This product is for in vitro diagnosis only.
  2. Avoid contamination when using the reagent, the container used must be clean, and please take necessary precautions, do not swallow, and avoid contact with skin and mucous membrane.
  3. Please dispose of the measured samples and waste liquids in accordance with the relevant national and local laws and regulations.
  4. When changing the reagent lot number, the calibration should be re-calibrated.


Modern Clinical Biochemical Tests, Zhang Xiuming and Li Jianzhai, 2001, P701

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