Selling unit: per 100ml
- Common name: Uric acid (UA) assay kit (uric acid enzyme method)
- English name: UA Reagent Kit (Uricase Method)
Single, 3:1, 4:1 (common ratio), other ratios need to be customized
This kit is used to determine the amount of UA in human serum, plasma or urine.
UA is the end product of purine catabolism in nucleic acids, which is excreted by the kidneys and excreted in the urine. Renal diseases such as acute and chronic nephritis, renal tuberculosis, gout, and diseases with excessive nucleic acid catabolism in the body, such as chronic leukemia and multiple myeloma, can increase the UA level; while the UA level in blood decreases when pernicious anemia and the application of ACTH, corticosteroids, aspirin and other drugs.
The urease coupled Trinder reaction was used. Urease first catalyzes the oxidation of uric acid to produce allantoin and hydrogen peroxide, and subsequently, hydrogen peroxide, 4-aminoantipyrine and TOOS form a red quinonimine compound by the action of POD (Trinder reaction), which causes an increase in absorbance at 548 nm, a change proportional to the UA concentration in the sample.
Reagent components included in the product.
*Components are not interchangeable in kits with different lot numbers.
*Reagent components not included in the product, but necessary for the test: outsourced normal and abnormal QCs and calibrators.
Storage Conditions And Expiration Date
The kit is stored at 2-8°C away from light and is valid for one year.
Reagents that have been opened are taken care not to be contaminated and the reagents are stabilized in the instrument compartment (2-8°C) for one month.
The reagents must not be frozen.
Hitachi 7180/7600; Olympus AU680/2700; Toshiba TBA120; Myriad BS2000M/480; Siemens ADVIA 1800/2400 series automatic biochemical analyzers.
Serum, heparin or EDTA anticoagulated plasma, urine. Uric acid in serum samples is stable for 5 days at 2 to 8 °C and for 6 months at -20 °C.
- Reagent preparation: liquid reagents are ready to use out of the bottle.
- test conditions: (different test instruments can be requested according to the different parameters on the machine)
Single reagent operation procedure.
Dual reagent operation procedure.
*Reagents and sample volumes can be increased or decreased in proportion to the requirements of different biochemical analyzers.
- Calibration procedure.
Randox calibration serum is recommended. the Randox calibration serum of choice is uric acid peroxidase combined with ascorbate oxidase 546 nm.
- QC control procedures.
It is recommended that each laboratory establish its own quality control system and select appropriate quality control products for quality control. The measured values of QC products should be within the specified range. If outside the specified range, it is necessary to take appropriate measures or contact the manufacturer.
UA content in the sample (μmol/L) =(ΔAsample – ΔAblank ) × Cstandard / (ΔAstandard – ΔAblank )
Positive Judgment Value
Serum and plasma: 90 to 420 μmol/L
It is recommended that each laboratory establish its own reference range of normal values.
Interpretation Of Test Results
The measurement of UA in serum, plasma or urine is only one of the indicators used by the clinician to make a diagnosis of the patient. The clinician must also make a comprehensive judgment based on the patient’s physical symptoms, medical history and other diagnostic items and diagnostic tools.
Limitations Of The Test Method
Ascorbic acid ≤ 20 mg/dL, bilirubin ≤ 20 mg/dL, hemoglobin ≤ 100 mg/dL, and celiac disease ≤ 500 mg/dL did not interfere with the assay.
Product Performance Index
Absorbance of reagent blank: wavelength 548 nm, optical diameter 1.0 cm, temperature 37°C, A0 ≤ 0.20
Analytical sensitivity: the kit tests 500 μmol/L of the test substance, the absorbance change value △A ≥ 0.02.
Linearity interval: test serum samples, reagent linearity 5 to 1190 μmol/L interval, linear correlation coefficient|r| should not be less than 0.990; in the 5 to 100 μmol/L interval, the absolute deviation of linearity should not exceed 15 μmol/L; in the (100 to 1190 μmol/L interval, the relative deviation of linearity should not exceed ±10%.
Precision: repeatability CV ≤ 4.0%; relative extreme difference between batches ≤ 6.0%.
Accuracy: Relative deviation ≤ 10%.
- 1. This product is for in vitro diagnosis only.
- 2. The reagents should avoid contamination, the containers used must be clean, and the unused reagents should be stored in the refrigerator at 2~8℃.
- When changing the reagent lot number, the calibration should be re-calibrated.
- 6. If the reagent accidentally splashes on the surface of human body such as skin, eyes, etc., it must be flushed with water, and if it is accidentally ingested, it needs to be treated in hospital.
- The third edition of the National Clinical Laboratory Practice (2006)
- Newman DJ, Price CP. Renal function and nitrogen
metabolites. In: Burtis CA, Ashwood ER, editors.Tietz Textbook of Clinical Chemistry. 3rd ed.Philadelphia: W.B SUAnders Company; 1999. p.1204-70.