Product Description
Food grade pullulanase for beer brewing industry ENZYMES.BIO PUL2
INTRODUCTION
This product is obtained by deep fermentation of Bacillus Licheniformis.It is widely used in manufacture of beer, starch sugar, monosodium glutamate and alcohol industry etc. The enzyme is a thermal stable debranching enzyme at low Ph (starch α-1,6-Glucosidase).It hydrolyzes the α-1,6–glucosidic linkages and effectively affect on branched chain starch and work in other enzymes (Glucoamylase ,Fungal Alpha- Amylase ).So ,it can quicken reaction speed and improve the content of glucoamylase and maltose
DEFINITION OF UNIT
1 unit of Pullulanase equals to the amount of enzyme needed to liberate 1µmol of reducing sugar (Calculated as glucose) from pullulan in one minute at pH5.8, 50℃
Characteristics
| Declared Activity | 2000 u/ml |
| Production Organism | Bacillus subtilis |
| Physical Form | Liquid |
| Color | Brown ,Color can vary from batch to batch. Color intensity is not an indication of enzyme activity |
| Odour | Normal microbial fermentation odour. |
SPECIFICATIONS
| ITEMS | Lower Limit | Upper Limit |
| enymes activity | 2000u/ml | |
| Lead | 5 mg/kg | |
| Arsenic | 3 mg/kg | |
| Total viable count | 50,000 CFU/g | |
| Coliform Bacteria | 30 CFU/g | |
| Escherichia coli | 10 CFU/g 3 MPN/g | |
| Salmonella | Not Detected/25g |
DOSAGE
In manufacture of beer: The recommended dosage is 0.4-0.8L of the enzyme preparation per ton of total raw materials. Pullulanase can reduce the content of amylopectin to produce dry beer. In manufacture of glucose: The recommended dosage is 0.35-0.6L per ton of dry starch. Pullulanse and glucoamylase together use in saccharification step. The usage of pullulanse can improve the yield of glucose by reducing the content of amylopectin, and can reduce the usage of glucoamylase. In manufacture of maltose: The recommended dosage is 1-2L per ton of dry starch. Pullulanase and Fungal Alpha-Amylase can together use in saccharification step. The usage of Pullulanse can improve the yield of maltose, and can produce maltose syrup with content of 70% maltose.
PACKAGE DAN STORAGE
Pactage
Packaging specification: 25L/drum; 1,125L/drum or as your request.
Storage
| Best Before | When stored as recommended, the product is best used within 6 months from date of delivery. |
| Shelf Life | 6 months at 25℃, activity remain ≥90% 12 months, activity remains ≥80% Increase dosage after shelf life. |
| Storage Conditions | This product should be stored in a cool and dry place in sealed container, avoiding insolation, high temperature and damp. The product has been formulated for optimal stability. Extended storage or adverse conditions such as higher temperature or higher humidity may lead to a higher dosage requirement. |
SAFETY
Enzyme preparations are proteins, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, consult a doctor.
