Question 1

What is lactase enzyme used for?

Accepted Answer

Lactase (β-galactosidase) hydrolyzes lactose into glucose and galactose. Industrial applications include: lactose-free milk production (UHT and pasteurized), ice cream (prevents crystallization, reduces added sugar), yogurt and fermented dairy (improved fermentation, residual lactose reduction), whey processing (improved fermentability and drying), and nutraceutical lactase supplements for lactose-intolerant consumers.

Question 2

What lactase activity grades are available?

Accepted Answer

We supply lactase powder in 10,000 ALU/g, 50,000 ALU/g, and 100,000 ALU/g activity grades, plus food-grade liquid lactase formulations for continuous dosing systems. Activity is expressed in ALU/g (acid lactase units at pH 4.5) or NLU/g (neutral lactase units at pH 6.5). CAS No.: 9031-11-2. All food-grade grades produced by controlled fermentation.

Question 3

What is the optimum pH and temperature for dairy lactase?

Accepted Answer

For UHT and pasteurized milk processing, neutral lactase (pH optimum 6.0–7.0) is used at refrigeration temperatures (4–10°C) for in-package hydrolysis over 24 hours, or at 35–40°C for accelerated in-tank processing. Acid lactase (pH optimum 4.0–5.0) is used for whey permeate and acid whey processing where pH is below 5.

Question 4

How long does it take to achieve complete lactose hydrolysis in milk?

Accepted Answer

With neutral lactase at 4°C (cold hydrolysis, typical for retail lactose-free milk): 12–24 hours in sealed containers. At 35–40°C (warm hydrolysis, industrial in-tank): 2–4 hours. Complete hydrolysis (>99%) is achievable at standard dosages. We provide application-specific dosage and process protocols with every order.

Question 5

Does lactase treatment affect the taste of milk?

Accepted Answer

Yes — in a positive way. Lactose hydrolysis produces glucose and galactose, which are intrinsically sweeter than lactose. Lactose-free milk is typically perceived as 30–40% sweeter than regular milk at the same solids content. This allows sugar reduction in dairy desserts and ice cream while maintaining desired sweetness.

Question 6

Can I order a small sample before buying in bulk?

Accepted Answer

Yes. Minimum order is 1 kg for powder grades. We supply the same specification at all order sizes. Certificate of analysis confirming ALU/g activity, pH optimum, and microbiological specifications ships with every batch.

Bulk Lactase Enzyme Supplier

How Lactase Works

Lactase Applications

Why Source from enzymes.bio?