Question 1

What is papain enzyme used for?

Accepted Answer

Papain is a plant-derived cysteine protease from papaya. Applications include: meat tenderization (beef, pork, poultry — injection or marinade), protein hydrolysate production (savory extracts, collagen peptides), brewing chill-proofing (haze protein hydrolysis), nutraceutical digestive enzyme supplements, and specialty feed processing for improved protein digestibility.

Question 2

What papain activity grades are available?

Accepted Answer

We supply papain from 80,000 U/g to 2,000,000 U/g in powder form. Standard grades include 100,000, 200,000, 650,000, 800,000, 1,000,000, 1,500,000, and 2,000,000 U/g, plus liquid papain preparations for continuous process dosing. CAS No.: 9001-73-4. All grades are off-white to cream powder derived from unripe papaya latex.

Question 3

What is the optimum temperature and pH for papain?

Accepted Answer

Papain is optimally active at 60–65°C and pH 5.0–7.0, but retains significant activity from pH 3.0–9.0. It remains active during cooking (50–70°C), enabling controlled tenderization, and is inactivated above 75°C. This thermal profile makes it ideal for meat marination where tenderization should complete during cooking rather than during storage.

Question 4

How does papain compare to bromelain for meat tenderization?

Accepted Answer

Both are plant cysteine proteases used for meat tenderization. Papain has higher maximum activity potential (up to 2,000,000 U/g vs 1,200,000 U/g for bromelain) and is typically less expensive at equivalent activity. Bromelain is preferred for applications requiring pH stability in acidic conditions. Both are used for brewing chill-proofing with similar effectiveness.

Question 5

Is papain suitable for clean-label food products?

Accepted Answer

Yes. Papain is a natural enzyme extracted from unripe papaya without genetic modification. It is classified as a processing aid in many jurisdictions and does not appear in final ingredient lists. It has GRAS status in the USA and is approved for use in food processing in major markets. Our supply is food-grade with complete CoA documentation.

Question 6

Can I order a small sample before buying in bulk?

Accepted Answer

Yes. Minimum order is 1 kg. We supply the same specification at all order sizes. Certificate of analysis with activity in U/g, pH optimum, microbiological specifications, and safety data sheet ships with every batch.

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