Glucose (GLU) (Hexokinase Method) Assay Kit – Bulk Reagents

US$42.00

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Selling unit: per 100ml

Product Name

  1. Common name: Glucose (GLU) assay kit (hexokinase method)
  2. English name: GLU Reagent Kit (Hexokinase Method)

Reagent Ratio

Single, 3:1, 4:1 (common ratio), other ratios need to be customized

Intended Use

This kit is used for the in vitro determination of GLU in serum, plasma, urine or cerebrospinal fluid.

GLU is the most predominant carbohydrate present in human blood. Pathologically increased: various kinds of diabetes; various other endocrine diseases: hyperthyroidism, anterior pituitary eosinophil adenoma, hyperadrenocorticism, pheochromocytoma, anterior pituitary basophilic hyperfunction; intracranial hypertension: cranial trauma, intracranial hemorrhage, meningitis, etc.; hyperglycemia caused by dehydration. Pathological lowering: pancreatic islet beta-cell tumor, hypoadrenocorticism, hypothyroidism, severe liver disease, overdose with hypoglycemic drugs, advanced uremia, severe malnutrition, liver disease, etc. Usually, various tolerance tests and stimulation tests are also performed along with GLU measurements when looking for the cause of these conditions.

Test Principle

In the presence of hexokinase (HK), GLU reacts with ATP to produce ADP and glucose-6-phosphate. Glucose-6-phosphate reacts with NAD+ to form NADH and gluconic acid 6-phosphate, catalyzed by glucose-6-phosphate dehydrogenase (G-6-PDH). NADH has maximum absorption at 340 nm, and the amount of GLU in the specimen can be calculated by measuring the amount of NADH produced compared to the calibration of GLU with the same treatment.

Main Components

Reagent components included in the product.

Glucose (GLU) Assay Kit (Hexokinase Method)

*Components are not interchangeable in kits with different lot numbers.

*Reagent components not included in the product, but necessary for the test: outsourced normal and abnormal QCs and calibrators.

Storage Conditions And Expiration Date

The kit is stored at 2-8°C away from light and is valid for one year.

Reagents that have been opened are taken care not to be contaminated and the reagents are stabilized in the instrument compartment (2-8°C) for one month.

The reagents must not be frozen.

Applicable Instruments

Hitachi 7180/7600; Olympus AU680/2700; Toshiba TBA120; Myriad BS2000M/480; Siemens ADVIA 1800/2400 series automatic biochemical analyzers.

Sample Request

Anticoagulate the plasma with serum, heparin or EDTA to avoid hemolysis. To reduce GLU loss due to glycolysis, serum should be separated from red blood cells as soon as possible. GLU in blood samples is stable for 1 day at room temperature (15-25°C) and 7 days at refrigerated (2-8°C) conditions. Urine samples are stable for 2h under refrigerated (2-8℃) conditions.

Test Method

  1. Reagent preparation: liquid reagents are ready to use out of the bottle.
  2. test conditions: (different test instruments can be requested according to the different parameters on the machine)

Glucose (GLU) Assay Kit (Hexokinase Method)

Single reagent operation procedure.

Glucose (GLU) Assay Kit (Hexokinase Method)

Dual reagent operation procedure.

Glucose (GLU) Assay Kit (Hexokinase Method)

*Reagents and sample volumes can be increased or decreased in proportion to the requirements of different biochemical analyzers as appropriate.

  1. Calibration procedure.

The Randox calibration serum is recommended, and the Randox calibration serum of choice is hexokinase.

  1. QC control procedures.

It is recommended that each laboratory establish its own quality control system and select appropriate quality control products for quality control. The measured values of QC products should be within the specified range. If outside the specified range, it is necessary to take appropriate measures or contact the manufacturer.

  1. Calculation.

GLU content in the sample (mmol/L) = (ΔAsample – ΔAblank ) × Cstandard / (ΔAstandard – ΔAblank )

Positive Judgment Value

Serum, plasma: 3.89-6.40 mmol/L

It is recommended that each laboratory establish its own reference range of normal values.

Interpretation Of Test Results

Measurement of GLU in serum, plasma, urine or cerebrospinal fluid is only one of the indicators used by the clinician to make a diagnosis of the patient.

Limitations Of The Test Method

Bilirubin ≤ 40 mg/dL and celiac ≤ 500 mg/dL had no effect on the assay.

Product Performance Index

Absorbance of reagent blank: wavelength 340 nm, optical diameter 1.0 cm, temperature 37°C, A0  ≤ 0.3.

Analytical sensitivity: the kit tests 1 mmol/L of the test substance, the absorbance change value ΔA ≥ 0.01.

Linearity interval: test serum samples, reagent linearity 0.2 to 25.0 mmol/L interval, linear correlation coefficient|r| should not be less than 0.990; in the 0.2 to 4.0 mmol/L interval, the absolute deviation of linearity should not exceed 0.4 mmol/L; in the (4.0 to 25.0 mmol/L interval, the relative deviation of linearity should not exceed ±10%.

Precision: repeatability CV ≤ 5%; relative extreme difference between batches ≤ 6%;

Accuracy: Relative deviation ≤ 10%.

Caution

  1. 1. This product is for in vitro diagnosis only.
  2. 2. Avoid contamination when using the reagent, the container used must be clean, and please take necessary precautions, do not swallow, and avoid contact with skin and mucous membrane.
  3. 3. Please dispose of the measured samples and waste liquids in accordance with the relevant national and local laws and regulations.
  4. 4. When changing the reagent lot number, please recalibrate.

Reference

  1. SCHMIDT, F. H. KLIN. WSCHR. 39. 819819. 1224;
  2. HOFFMEISTER H. N. JUNGE, B. Z. KLIN. CHEM, KLIN BIOCHEM. 8 (1970) 6.13
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