Glutamate Dehydrogenase (GLDH ) Assay Kit – Bulk Reagents

US$58.00

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Selling unit: per 100ml

Product Name

Common name: Glutamate dehydrogenase (GLDH ) assay kit (rate method)

English Name.GLDH Reagent Kit (Kinetic Method)

Reagent Ratio

Single reagent, 3:1, 4:1, 5:1, commonly used single reagent, double reagent according to the ratio of custom

Product Use

Used to determine the activity of glutamate dehydrogenase (GLDH) in human serum and plasma.

Glutamate dehydrogenase is a mitochondrial enzyme present in all tissues. in vivo GLDH has the highest content in the liver, mainly in hepatic lobular cells, and the enzyme activity is extremely low in normal serum. gLDH is a liver-specific mitochondrial enzyme that enters the blood when hepatocytes are necrotic, and the level of serum GLDH can reflect the degree of hepatocyte damage.

Detection Principle

GLDH

α-ketoglutarate + NH3 +NADH ───→ Glutamic acid +NAD+

The rate of change of NADH absorbance per minute was measured at 340 nm and its content was calculated.

Reagent Composition

Glutamate dehydrogenase (GLDH ) assay kit

Storage Expiration Date

For opening the kit can be stable for one year when stored at 2~8℃ away from light; reagents can be stable for one month when stored at 2~8℃ away from light after opening the bottle. Reagents should not be frozen.

Sample Request

For serum or heparin/EDTA anticoagulated plasma specimens, the serum should be separated promptly after blood collection to avoid hemolysis. Specimens should be separated by centrifugation immediately after blood collection, and samples can be stored at room temperature (15-25°C) for 7 days, 2-8°C for 7 days, and -20°C for 4 weeks.

Measurement Method

  1. Test conditions: (different test instruments can be requested according to the different parameters on the machine)

Glutamate dehydrogenase (GLDH ) assay kit

  1. Operation steps.

Glutamate dehydrogenase (GLDH ) assay kit

Single reagent operation: liquid single reagent, can be used directly.

Dual reagent operation: both reagent 1 and reagent 2 are liquid products and can be used directly.

Glutamate dehydrogenase (GLDH ) assay kit

*Reagents and sample volumes can be increased or decreased in proportion to the requirements of different biochemical analyzers.

  1. Calibration procedure.

Randox calibration serum is recommended.

  1. QC control procedures.

It is recommended that each laboratory establish its own quality control system and select appropriate quality control products for quality control. The measured values of QC products should be within the specified range. If outside the specified range, it is necessary to take appropriate measures or contact the manufacturer.

  1. Calculation.

GLDH activity (U/L) in the sample = (ΔAsample /min-ΔAblank /min) × Cstandard / (ΔAstandard /min-ΔAblank /min).

Reference Range

Serum and plasma: Adult male 0-7 U/L Adult female: 0-5 U/L

It is recommended that each laboratory establish its own reference range of normal values.

Anti-interference

Ascorbic acid ≤ 40 mg/dl, bilirubin ≤ 40 mg/dl, hemoglobin ≤ 500 mg/dl, triglycerides ≤ 2000 mg/dl did not interfere with the determination.

Performance Indicators

Absorbance of reagent blank: wavelength 340 nm, optical diameter 1.0 cm, temperature 37°C± 1°C, A0  ≥ 0.8. A0 < 0.8 when disabled or replaced with new reagent.

Reagent blank absorbance change rate: wavelength 340 nm, optical diameter 1.0 cm, temperature 37 ℃± 1 ℃, the measured reagent absorbance change value ︱ △A/min ︱ ≤ 0.0005.

Analytical sensitivity: the kit tests 10 U/L of the test substance, the absorbance change rate ΔA/min ≥0.001.

Linearity range: 2~120 U/L (Judgment basis: r ≥ 0.990). If out of linear range, please measure after dilution with saline and multiply the result by the dilution factor.

Precision: intra-batch coefficient of variation ≤ 6.0 %; inter-batch coefficient of variation ≤ 8.0 %.

Accuracy: Absolute value of relative deviation ≤ 10 %.

Correlation: Comparison with similar products: n = 120, r = 0.9941, y = 0.9738x + 0.3936 (y: our reagent; x: other reagents)

Caution

  1. This product is for in vitro diagnosis only, please use it correctly according to the instruction manual.

2、When using the reagent, avoid contamination, the container used must be clean, and please take necessary precautions, do not swallow, and avoid contact with skin and mucous membrane.

3、Please dispose of the measured samples and waste liquid in accordance with the relevant national and local laws and regulations.

4、Do not mix R1 and R2 into a single reagent, and do not exchange reagents with different batch numbers.

5、Please re-calibrate when changing the reagent lot number.

6, the theoretical K value is for reference only, and the actual K value may not be the same, so it is recommended to use the calibration serum to set the standard, high fat or jaundice samples should be diluted and then tested.

Reference

  1. Schmidt, E. and Schmidt, F.W. In: Method of Enzymatic Analysis, 3rd ed. H.K. Bergmeyer, J. Bergmeyer, and M. Grosse, Eds. Weirheim, Verlag Chemie, ( Weirheim, Verlag Chemie, ( 1983), 3: 216-227.
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