Glycyl Prolyl Dipeptide Aminopeptidase (GPDA) Assay Kit – Bulk Reagents

$167.00

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Category:
1667

Selling unit: per 100ml

Product Name

Common name: Glycyl prolyl dipeptide aminopeptidase (GPDA ) assay kit (enzyme rate method)

English Name.GPDA Reagent Kit (Enzyme Kinetic Method)

Reagent Ratio

3:1, 4:1, 5:1, common ratio 4:1, other ratios need to be customized

Product Use

For the determination of glycyl prolyl dipeptide aminopeptidase (GPDA) activity in human serum and plasma.

The clinical application of the program is helpful in the diagnosis and monitoring of liver cancer and gastric cancer; the determination of GPDA in urine has important clinical value for nephrotic syndrome, uremia, acute and chronic glomerulonephritis, etc.

Detection Principle

Under alkalinity, GPDA catalyzes the hydrolysis of glycinyl proline p-nitroanilide to produce glycinyl proline and yellow p-nitroanilide, the latter causing an increase in absorbance at 404 nm (400-420 nm), and the rate of increase in absorbance is proportional to GPDA activity.

Reagent Composition

Glycyl prolyl dipeptide aminopeptidase (GPDA) assay kit

Storage Expiration Date

Unopened kits can be stable for one year when stored at 2-8℃ away from light; reagents can be stable for one month when stored at 2-8℃ away from light after opening. Reagents should not be frozen.

Sample Request

The most suitable sample is fresh non-hemolyzed serum or plasma. Plasma can be anticoagulated with heparin, EDTA and citrate. The GPDA in the sample is stable for 2 days at 2 to 8°C and 30 days at -20°C.

Measurement Method

  1. Test conditions: (different test instruments can be requested according to the different parameters on the machine)

Glycyl prolyl dipeptide aminopeptidase (GPDA) assay kit

  1. Operation steps.

Glycyl prolyl dipeptide aminopeptidase (GPDA) assay kit

Reagents and sample volumes can be increased or decreased in proportion to the requirements of different biochemical analyzers.

  1. Calibration procedure.

The use of matching calibrators is recommended.

  1. QC control procedures.

It is recommended that each laboratory establish its own quality control system and select appropriate quality control products for quality control. The measured values of QC products should be within the specified range. If outside the specified range, it is necessary to take appropriate measures or contact the manufacturer.

  1. Calculation.

GPDA activity (U/L) in the sample =(ΔAsample – ΔAblank ) × Cstandard / (ΔAstandard – ΔAblank )

Reference Range

44 to 116 U/L

It is recommended that each laboratory establish its own reference range of normal values.

Anti-interference

Ascorbic acid ≤ 30 mg/dl, free bilirubin ≤ 684 μmol/L (40 mg/dl), conjugated bilirubin ≤ 684 μmol/L (40 mg/dl), hemoglobin ≤ 400 mg/dl, and triglycerides ≤ 2000 mg/dl did not interfere with the assay.

Performance Indicators

Absorbance of reagent blank: wavelength 404 nm, optical diameter 1.0 cm, temperature 37°C± 1°C, A0  ≤ 0.5. A0 > 0.5 when disabled or replaced with new reagent.

Reagent blank absorbance change rate: wavelength 404 nm, optical diameter 1.0 cm, temperature 37 ℃± 1 ℃, the measured reagent absorbance change value ︱ △A/min ︱ ≤ 0.001.

Analytical sensitivity: the absorbance change rate ΔA/min ≥0.02 when the kit tests 100 U/L DUT.

Linearity range: 4~400 U/L (Judgment basis: r ≥ 0.975). If out of linear range, please measure after dilution with saline and multiply the result by the dilution factor.

Precision: intra-batch coefficient of variation ≤ 5.0 %; inter-batch coefficient of variation ≤ 6.0 %.

Accuracy: Absolute value of relative deviation ≤ 10 %.

Correlation: Comparison with similar products: n = 120, r = 0.9949, y = 1.0036x + 0.9195 (y: our reagent; x: other reagents)

Caution

  1. 1. This product is for in vitro diagnosis only, please use it correctly according to the instruction manual.

2、When using the reagent, avoid contamination, the container used must be clean, and please take necessary precautions, do not swallow, and avoid contact with skin and mucous membrane.

3、Please dispose of the measured samples and waste liquid in accordance with the relevant national and local laws and regulations.

4、Do not mix R1 and R2 into a single reagent, and do not exchange reagents with different batch numbers.

5、Please re-calibrate when changing the reagent lot number.

  1. 6. Theoretical K values are for reference only and may not be the same as the actual K values, so it is recommended to use calibrated serum to set the standard.

Reference

  1. Kato T, et al. Excretion of X-prolyl dipeptidyl aminopeptidase in human urine as determined with a new fluorogenic substrate, Clin Chem, (1978), 24(7) : 1163-1166.
  2. Nagatsu T, et al. New chromogenic substrate for X-prolyl dipeptidyl aminopeptidase, Anal Bioch, (1978), 74: 466-477.
  3. Zhang Z, et al. Advances in the study of glycyl prolyl dipeptide aminopeptidase, Journal of Clinical Investigation, (1997), 15(1): 57-60.
  4. Kojima J, et al. Serum glycyl praline dipeptidyl aminopeptidase activity in human hepatic cancer, Clin Chem Acta, (1997), 93: 181-187.
  5. Hino M, et al. X-prolyl dipeptidyl aminopeptidase activity with X-proline-P-nitraanilides as substrate in normal and pathological human sera, Clin Chem, (1976), 22(8): 1256-1261.
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