Carbon Dioxide (CO2 ) Assay Kit – Bulk Reagents


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Selling unit: per 100ml

Product Name

Common name: Carbon dioxide (CO2 ) assay kit (enzyme method)

English name: CO2 Reagent Kit(Enzymatic Method)

Package Specification

Single reagent

Intended Use

This kit is used to determine the amount of CO2 in human serum or plasma.

CO2 is usually measured in combination with tests for glucose, urea, potassium, sodium, and chloride to determine metabolic or respiratory-induced imbalances in acid-base balance. Increased CO2 in plasma is commonly associated with pyloric obstruction, Cushing’s syndrome, and overdose of alkaline drugs. Respiratory acidosis: such as pulmonary heart disease, central respiratory depression, respiratory muscle paralysis, emphysema, bronchiectasis and pneumothorax. Reduced plasma CO2 is common in severe diarrhea, renal failure, diabetic ketosis, infectious shock, and overdose of acidic drugs. Chronic respiratory alkalosis: PCO2 is reduced in the alveoli and renal tubular compensatory HCO3 excretion is increased due to prolonged respiratory rate increase.

Test Principle

Phosphoenolpyruvate carboxylase (PEPC) catalyzes the bicarbonate in the sample to produce oxaloacetate and phosphate; oxaloacetate is reduced to malate catalyzed by malate dehydrogenase (MDH) while NADH is oxidized to NAD+. The amount of bicarbonate in the sample can be calculated by measuring the decrease in absorbance at 404 nm.

Main Components

Reagent components included in the product.

Carbon dioxide (CO2 ) assay kit

*Components are not interchangeable in kits with different lot numbers.

*Reagent components not included in the product, but necessary for the test: outsourced normal and abnormal QCs and calibrators.

Storage Conditions And Expiration Date

The kit is stored at 2-8°C away from light and is valid for one year.

Reagents that have been opened are careful not to be contaminated, and reagents are stable in the instrument compartment (2-8°C) for one month.

The reagents must not be frozen.

Applicable Instruments

Hitachi 7180/7600; Olympus AU680/2700; Toshiba TBA120; Myriad BS2000M/480; Siemens ADVIA 1800/2400 series automatic biochemical analyzers.

Sample Request

Serum or heparin-anticoagulated plasma.

Samples should be tested within 1 hour of refrigeration. During collection and handling, tighten the cap as much as possible to reduce exposure to air, otherwise CO2 in the sample will diffuse and lead to false results (up to 6 mmol/hr).

Test Method

  1. Reagent preparation: liquid reagents are ready to use out of the bottle.
  2. test conditions: (different test instruments can be requested according to the different parameters on the machine)

Carbon dioxide (CO2 ) assay kit

Operation steps.

Carbon dioxide (CO2 ) assay kit

Reagents and sample volumes can be increased or decreased in proportion to the requirements of different biochemical analyzers.

  1. Calibration procedure.

The Randox calibration serum is recommended and the method of choice is the enzymatic method.

  1. QC control procedures.

It is recommended that each laboratory establish its own quality control system and select appropriate quality control products for quality control. The measured values of QC products should be within the specified range. If outside the specified range, it is necessary to take appropriate measures or contact the manufacturer.

  1. Calculation.

CO2 concentration in the sample (mmol/L) = (A样品 -A空白 )/min ×C标准 /(A标准 -A空白 )/min

Positive Judgment Value Or Reference Interval

Adults: 20 to 29 mmol/L

It is recommended that each laboratory establish its own reference range of normal values.

Interpretation Of Test Results

The assay of CO2 in serum or plasma is only one of the indicators used by the clinician to make a diagnosis of the patient. The clinician must also make a comprehensive judgment based on the patient’s physical symptoms, medical history, and other diagnostic items and tools.

Limitations Of The Test Method

Bilirubin ≤ 40 mg/dL, hemoglobin ≤ 500 mg/dL and celiac ≤ 500 mg/dL did not interfere with the assay.

Product Performance Index

Absorbance of reagent blank: wavelength 404 nm, optical diameter 1.0 cm, temperature 37°C, A0  ≥ 0.8.

Absorbance change rate of reagent blank: △A/min ≤ 0.001 at wavelength 404 nm and optical diameter 1.0 cm.

Analytical sensitivity: The kit tests 10 mmol/L of the test substance with an absorbance change rate ΔA/min ≥ 0.004.

Linearity range: test serum samples, reagent linearity in the interval of 5.0~40.0 mmol /L, linear correlation coefficient|r| should not be less than 0.975; in the interval of 5.0~30.0 mmol/L, the absolute deviation of linearity should not exceed 3.0 mmol/L; in the interval of (30.0~40.0 mmol/L, the relative deviation of linearity should not exceed ± 10%.

Precision: repeatability CV ≤ 6%; relative extreme difference between batches ≤ 10%.

Accuracy: Relative deviation ≤ 10%.


  1. This product is for in vitro diagnosis only.
  2. Avoid contamination when using the reagent, the container used must be clean, and please take necessary precautions, do not swallow, and avoid contact with skin and mucous membrane.
  3. Please dispose of the measured samples and waste liquids in accordance with the relevant national and local laws and regulations.
  4. Do not mix R1 and R2 into a single reagent.
  5. When changing the reagent lot number, please recalibrate.
  6. Samples and reagents should be kept out of contact with air as much as possible and caps should be tightened after reagent use to avoid CO2contamination.
  7. Lipid blood samples cannot be used for this assay.


  1. Tietz, N.W. (Ed.), Fundamentals of Clinical Chemistry, W.B. Saunders Co.
  2. Norris, K.A., Atkinson, A.R., Smith, W.G., Clin. Chem. 21 (1975).
  3. US Patent #5,801,006
  4. Young, D.S., Effects of Drugs on Clinical LaboratoryTests, AACC Press, Third Edition, Washington (1990).
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