Retinol Binding Protein (RBP) (Immunoturbidimetric Assay) Assay Kit – Bulk Reagents

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Selling unit: per 100ml

Product Name

Common name: Retinol binding protein (RBP ) assay kit (immunoturbidimetric method)

English Name.RBP Reagent Kit (Immunoturbidimetric Method)

Reagent Ratio

3:1, 4:1, 5:1, common ratio 4:1, other ratios need to be customized

Intended Use

This kit is used to determine the level of human serum RBP.

RBP is a sensitive indicator of the nutritional status of the organism, especially protein-calorie malnutrition. Visceral protein is used as a traditional laboratory indicator of protein-energy malnutrition, while depending on the nutritional status retinol-binding protein RBP can provide a faster response.

RBP is mainly synthesized in the liver, so the decrease and increase of serum RBP is related to liver disease and influenced by the presence and severity of liver disease. In liver disease, serum RBP levels are significantly reduced in cirrhosis and in both acute and chronic hepatitis.

RBP can be used as an early diagnostic indicator of renal tubular injury. Retinol-binding protein RBP is stable in urine, not easily decomposed, and not disturbed by pH and blood pressure. In renal proximal tubular injury, its urinary excretion increases significantly, so the increase in urinary RBP excretion can be used as a marker of renal proximal tubular injury. When renal filtration function is reduced, blood RBP shows increased concentration due to storage. The concentration of RBP in blood or urine can be used as an ideal indicator of renal function in clinical practice.

Test Principle

The RBP in the serum binds to the antibody in the appropriate liquid phase to form an antigen-antibody complex, resulting in a change in turbidity that is proportional to the amount of RBP in the sample. The turbidity is measured and compared with the standard serum at multiple points of calibration, and the amount of RBP in the sample serum is obtained.

Main Components

Reagent components included in the product.

Retinol Binding Protein (RBP) Assay Kit & Bulk Reagents

*Components are not interchangeable in kits with different lot numbers.

*Reagent components not included in the product, but necessary for the test: outsourced normal and abnormal QCs and calibrators.

Storage Conditions And Expiration Date

The kit is stored at 2-8°C away from light and is valid for one year.

Reagents that have been opened are taken care not to be contaminated and the reagents are stabilized in the instrument compartment (2-8°C) for one month.

The reagents must not be frozen.

Applicable Instruments

Hitachi 7180/7600; Olympus AU680/2700; Toshiba TBA120; Myriad BS2000M/480; Siemens ADVIA 1800/2400 series automatic biochemical analyzers.

Sample Request

Fresh serum, if stored for a long time, should be stored frozen at – 20°C.

Test Method

  1. Reagent preparation: liquid reagents are ready to use out of the bottle.
  2. test conditions: (different test instruments can be requested according to the different parameters on the machine)

Retinol Binding Protein (RBP) Assay Kit & Bulk Reagents

Operation steps.

Retinol Binding Protein (RBP) Assay Kit & Bulk Reagents

*Reagents and sample volumes can be increased or decreased in proportion to the requirements of different biochemical analyzers as appropriate.

  1. Calibration procedure.

The use of matching calibrators is recommended.

  1. QC control procedures.

It is recommended that each laboratory establish its own quality control system and select appropriate quality control products for quality control. The measured values of QC products should be within the specified range. If outside the specified range, it is necessary to take appropriate measures or contact the manufacturer.

  1. Calculation.

Using multi-point calibration, a standard curve was made with the calibrator concentration against the corresponding ΔA. The RBP concentration in the sample was read off the standard curve by the ΔA of the sample.

Positive Judgment Value Or Reference Interval

Retinol Binding Protein (RBP) Assay Kit & Bulk Reagents

It is recommended that each laboratory establish its own reference range of normal values.

Interpretation Of Test Results

RBP measurement in serum is only one of the indicators used by clinicians to diagnose patients. Clinicians must also make a comprehensive judgment based on the patient’s physical symptoms, medical history and other diagnostic items and diagnostic tools.

Limitations Of The Test Method

Bilirubin ≤ 50 mg/dl (855 µmol/L), hemoglobin ≤ 500 mg/dl (5 g/L), and internal lipids ≤ 0.125 % (equivalent to celiac particles ≤ 125 mg/dl (1.25 g/L)) did not interfere with the assay.

Product Performance Index

Absorbance of reagent blank: wavelength 340 nm, optical diameter 1.0 cm, temperature 37°C± 1°C, A0  ≤ 0.3. A0 > 0.3 when disabled or replaced with new reagent.

Analytical sensitivity: the absorbance change value △A ≥ 0.05 when the kit tests 70 mg/L of the test substance.

Linearity interval: test serum samples, reagent linearity in the interval of 3.5-250 mg/L, the linear correlation coefficient |r| should not be less than 0.990; in the interval of 3.5~70 mg/L, the absolute deviation of linearity should not exceed 2 mg/L; in the interval of (70- 250 mg/L, the relative deviation of linearity should not exceed ±10%.

Precision: repeatability CV ≤ 5%; relative extreme difference between batches ≤ 10%.

Accuracy: Relative deviation ≤ 10%.

Caution

  1. This product is for in vitro diagnosis only.
  2. To avoid absorption of proteins in glass containers, use plastic containers.
  3. 3. Avoid the formation of bubbles when handling samples and reagents and confirm that there are no air bubbles in the samples and reagents during the test.
  4. 4. Even samples that are negative for HBS antigen, HIV antigen, and HCV antibodies should be handled carefully and safely, as there may be a potential risk of infection.
  5. 5. The buffer solution and antiserum reagent contain sodium azide, which can react strongly with copper, lead and other metals to form azide metal, so please dilute the waste solution fully and flush the drain when discarded to avoid residue in the drain.

Reference

1 . Kanai M. et al: J Clin Invest,47,2025-2044 (1968).

  1. Kanai M. et al: Nippon Rinsho 57.279-281 (1999).
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