Alkaline protease, also known as Bacillus licheniformis protease, is a kind of Endo protease, which is produced by deep fermentation of Bacillus licheniformis 2709 and refined by microfiltration, ultrafiltration and vacuum freeze-drying. It consists of 275 and 274 amino acid residues, forming a polypeptide chain with a molecular weight of 27300 da.
Mechanism of action
The active center of alkaline protease contains serine, so it is called serine protease. It can hydrolyze not only peptide bond, but also amide bond, ester bond, trans ester and trans peptide. It can hydrolyze protein to polypeptide or amino acid, and is widely used in detergent, food, medical, brewing, silk, leather and other industries.
The product is easily soluble in water, and the water solution is a clear light brown liquid.
Product Name: alkaline protease, alkaline enzyme main components: alkaline protease, glucose product specification: 100000-1.5 million U / g (customizable) product characteristics; Light brown powder storage method: room temperature drying, dark storage, shelf life: 12 months
1. Food industry
Alkaline protease is a kind of non-toxic and non side effect protein, which belongs to serine type endoprotease. It can hydrolyze protein macromolecular peptide chain to produce small molecular polypeptide or amino acid in food industry, and form protein hydrolysate with unique flavor.
2. Washing industry
Alkaline protease has been successfully used in detergent enzyme industry. It can be added to ordinary washing powder, concentrated washing powder and liquid detergent. It can be used for household laundry and industrial laundry. It can effectively remove blood stains, eggs, dairy products, meat juice, vegetable juice and other egg stains. In addition, it can also be used as a medical reagent for enzyme cleaning biochemical instruments.
3. Biological research
In the field of biotechnology, basic protein can be used as a tool enzyme to remove proteins (including nucleases) in the process of nucleic acid purification, without degradation of DNA, thus avoiding the destruction of DNA integrity.