Dual-Enzyme Granular Detergent Enzyme – Protease & Amylase Blend for Protein and Starch Stain Removal TIO
Dual-Enzyme Granular Detergent Enzyme – Protease & Amylase Blend TIO is a concentrated two-enzyme formulation developed for modern detergent and cleaning applications. It combines alkaline protease and alkaline amylase in a stable granular form to efficiently remove both protein-based stains and starch-based residues. The synergistic action of these two enzymes significantly enhances detergent performance by breaking down complex organic soils that are difficult to remove using surfactants alone.
This enzyme blend is suitable for incorporation into powder laundry detergents, automatic dishwasher detergents, institutional laundry products, industrial cleaning formulations, and specialty cleaning products. The free-flowing granules are easy to handle, blend uniformly into dry detergent formulations, and provide excellent storage stability while minimizing dust generation during manufacturing.
Whether used in household detergents or professional cleaning products, this dual-enzyme system helps deliver brighter fabrics, cleaner tableware, improved stain removal, and enhanced washing efficiency under a wide range of washing conditions.
Product Description
Protein and starch are two of the most common components found in everyday stains. Food residues, body soils, dairy products, sauces, baby food, grains, and many cooked foods contain complex mixtures of proteins and carbohydrates that strongly adhere to fabrics, dishes, and hard surfaces.
Traditional detergent ingredients such as surfactants and builders can loosen these soils, but enzymes provide an additional biological cleaning mechanism by selectively breaking down the stain molecules themselves.
The protease component hydrolyzes proteins into soluble peptides and amino acids, allowing stubborn protein stains to detach more easily during washing. Meanwhile, the amylase component breaks starch molecules into smaller soluble sugars and dextrins, helping eliminate starch deposits that often trap other types of dirt.
By combining these two enzyme activities into a single product, TIO offers broad-spectrum cleaning performance for a wide variety of household and commercial washing applications.
Key Features
- Dual-enzyme system combining alkaline protease and alkaline amylase
- Excellent removal of protein-based stains
- Highly effective against starch-containing food residues
- Suitable for powder laundry detergent formulations
- Ideal for automatic dishwasher detergents
- Broad operating pH compatibility
- Effective over moderate washing temperatures
- Granular form for convenient handling and blending
- Excellent formulation compatibility
- Suitable for household, commercial, and industrial detergent products
Typical Applications
- Powder laundry detergents
- Laundry detergent concentrates
- Automatic dishwasher detergents
- Commercial laundry detergents
- Industrial cleaning formulations
- Institutional cleaning products
- Textile washing formulations
- Cleaning products for hotels, hospitals, restaurants, and food service facilities
Typical Stains Removed
Protein-Based Stains
- Blood
- Egg
- Milk
- Sweat
- Meat juices
- Dairy residues
- Food protein deposits
- Body soils
Starch-Based Stains
- Rice
- Potatoes
- Pasta
- Bread
- Flour residues
- Sauces
- Baby food
- Desserts
- Cereal residues
- Cooked grain residues
Product Specifications
| Product Name | Dual-Enzyme Granular Detergent Enzyme – Protease & Amylase Blend TIO |
| Product Model | TIO |
| Enzyme Composition | Alkaline Protease + Alkaline Amylase |
| Appearance | Free-flowing granules |
| Color | Light beige to yellowish granules |
| Odor | Slight fermentation odor |
| Product Form | Granular |
| Packaging | Supplied in 1 kg/bag, sealed food-grade aluminum foil bag. Please note that the product image is for illustration purposes. Actual packaging may differ. |
As enzyme products are produced through biological fermentation, slight color variations between production batches may occur. These natural variations do not affect enzyme activity or product performance.
Operating Conditions
| Recommended Temperature | 20–60°C |
| Recommended pH Range | 7.0–11.5 |
The enzyme blend performs efficiently across the alkaline pH range commonly used in laundry detergents and dishwasher detergents. Actual cleaning performance depends on detergent formulation, washing temperature, washing time, surfactant system, builders, bleaching agents, water hardness, and the nature of the stains.
Recommended Dosage
The optimum dosage depends on detergent formulation, desired cleaning performance, enzyme activity requirements, and production conditions. Laboratory formulation testing is recommended to determine the ideal addition level.
| Powder Laundry Detergents | 0.3–1.3% (w/w) |
| Automatic Dishwasher Detergents | 2.5–6.0% (w/w) |
Mechanism of Action
Protease Activity
Protease catalyzes the hydrolysis of protein molecules into smaller peptides and amino acids. These smaller molecules dissolve more readily in the wash solution, allowing stubborn protein stains to be removed efficiently during the washing process.
Protease is particularly effective for removing stains originating from blood, eggs, dairy products, meat, sweat, and other protein-containing materials.
Amylase Activity
Amylase hydrolyzes starch molecules into soluble dextrins and simple sugars. Since starch often acts as a binding material that traps dirt and proteins on fabrics and dishes, breaking down starch significantly improves the overall cleaning efficiency of the detergent formulation.
Amylase is especially effective on rice, pasta, potatoes, bread, flour, cereals, sauces, and many processed food residues.
Synergistic Cleaning Performance
Most household stains contain multiple organic components rather than a single substance. Food residues frequently consist of proteins, starches, fats, sugars, and pigments combined together. A single enzyme often targets only one type of stain, whereas the combination of protease and amylase provides broader cleaning coverage.
The synergistic action of these two enzymes improves stain degradation, allowing surfactants to penetrate and remove soils more efficiently. This results in improved washing performance while maintaining effective cleaning at moderate temperatures.
Advantages for Detergent Formulators
- Broad stain-removal spectrum
- Improved cleaning performance against mixed food soils
- Compatible with most alkaline detergent systems
- Easy incorporation into powder formulations
- Excellent storage stability
- Low-dust granular technology
- Supports high-performance detergent formulations
- Suitable for household and institutional detergent products
Formulation Recommendations
- Suitable for dry blending into powder detergent formulations.
- Compatibility testing is recommended with surfactants, bleaching agents, builders, fragrances, and other detergent additives.
- Avoid prolonged exposure to excessive moisture during manufacturing.
- Store sealed until use to maintain enzyme stability.
- Optimize dosage through laboratory formulation trials for maximum cleaning performance.
Storage Conditions
Store in the original sealed package in a cool, dry environment.
- Recommended storage temperature: 2–25°C
- Keep away from direct sunlight.
- Avoid excessive humidity.
- Avoid prolonged exposure to elevated temperatures.
- Keep the package tightly sealed after opening.
Proper storage conditions help preserve enzyme activity and maximize product shelf life.
Safety Information
This product contains active enzymes. Like all enzyme preparations, it should be handled according to good industrial hygiene practices.
- Avoid inhalation of enzyme dust.
- Avoid unnecessary contact with skin and eyes.
- Wear appropriate gloves, protective clothing, eye protection, and respiratory protection when handling large quantities.
- Wash hands thoroughly after handling.
- If skin contact occurs, wash thoroughly with water.
- If the product enters the eyes, rinse immediately with plenty of clean water and seek medical advice if irritation persists.




