Three in One Liquid Detergent Enzyme Blend with Protease Amylase and Lipase for Laundry Detergents Laundry Pods and Multi Stain Removal HIOL
Three in One Liquid Detergent Enzyme Blend HIOL is a high-performance multi-enzyme formulation developed for liquid laundry detergents, laundry pods, and textile cleaning applications. This advanced liquid enzyme blend combines protease, amylase, and lipase to provide effective cleaning against protein-based, starch-based, and grease-based stains.
The three enzymes work together to break down different types of organic contamination into smaller, more soluble or dispersible substances, helping improve stain removal during washing. HIOL supports efficient cleaning performance while helping maintain fabric cleanliness and appearance.
Designed for neutral to alkaline detergent formulations, HIOL operates within a recommended pH range of 7.0 to 11.0 and a temperature range of 25 to 60°C. Its liquid form makes it suitable for incorporation into compatible laundry detergent formulations.
Key Features and Benefits
- Three Enzyme Technology: Combines protease, amylase, and lipase for comprehensive organic stain removal.
- Enhanced Cleaning Performance: Helps remove protein, starch, grease, and oil-based stains.
- Synergistic Enzyme Action: Multiple enzymes target different components of complex stains.
- Fabric Care: Supports fabric cleanliness and brightness by removing accumulated organic residues.
- Broad pH Compatibility: Suitable for detergent applications operating at pH 7.0 to 11.0.
- Versatile Temperature Range: Designed for washing conditions between 25 and 60°C.
- Liquid Formulation: Convenient for incorporation into compatible liquid detergents and laundry pods.
- Enzyme Based Cleaning: Uses targeted enzymatic hydrolysis to support effective detergent performance.
Product Specifications
| Product Name | Three in One Liquid Detergent Enzyme Blend |
| Product Code | HIOL |
| Enzyme Components | Protease, Amylase, Lipase |
| Physical Form | Liquid |
| Appearance | Light Brown Liquid |
| Odor | Slight Fermentation Odor |
| Operating Temperature | 25 to 60°C |
| Operating pH | 7.0 to 11.0 |
| Recommended Dosage | 0.2 to 1.0% w/w of Detergent Weight |
| Packaging | 1 kg bottle (supplied in an industrial-grade plain bottle). Please note that the product image is for illustration purposes. Actual packaging may differ. |
| Storage Temperature | 2 to 25°C |
| Applications | Liquid Laundry Detergents, Laundry Pods, Textile Washing |
Note: Minor color variations may occur between production batches due to differences in raw materials. Such variations do not necessarily affect product performance.
Enzyme Composition and Mechanism of Action
Protease for Protein Stain Removal
Protease catalyzes the hydrolysis of protein molecules into smaller peptides and soluble fragments, allowing protein-based stains to be more easily dispersed and removed during washing.
It is particularly useful for removing blood, milk, egg, grass-related protein contamination, and other protein-containing food stains.
Protease helps improve detergent cleaning efficiency by breaking down protein deposits that may adhere strongly to textile fibers.
Amylase for Starch Stain Removal
Amylase hydrolyzes starch molecules into smaller carbohydrate fragments, including soluble dextrins and shorter saccharide chains. These substances can be more easily removed by the washing solution.
It is effective against starch-containing stains from sauces, gravies, baby food, desserts, rice, pasta, and other carbohydrate-rich food residues.
Amylase complements protease by targeting starch deposits that cannot be directly hydrolyzed by protein-degrading enzymes.
Lipase for Grease and Oil Stain Removal
Lipase catalyzes the hydrolysis of triglycerides into glycerol and fatty acids under suitable washing conditions.
This enzymatic reaction helps break down fat-based contamination and supports the removal of cooking oils, animal fats, greasy food residues, body oils, and suitable cosmetic or skincare stains.
Lipase works together with detergent surfactants to facilitate the dispersion and removal of oily residues from washable fabrics.
Multi Enzyme Synergistic Cleaning
Many household stains contain combinations of proteins, starches, and fats. A detergent containing only one enzyme may target only part of these complex stains.
HIOL combines three complementary enzymatic activities to provide broader stain removal coverage. Protease breaks down proteins, amylase hydrolyzes starch, and lipase degrades suitable fats and oils.
The resulting smaller molecules can be more easily dispersed into the washing solution and removed through the combined action of surfactants, water, and mechanical washing.
Operating Conditions
| Temperature Range | 25 to 60°C |
| pH Range | 7.0 to 11.0 |
| Recommended Addition | 0.2 to 1.0% w/w |
HIOL is designed for neutral to alkaline washing conditions. Enzyme performance depends on temperature, pH, detergent composition, contact time, and stain characteristics.
Excessive heat, unsuitable pH conditions, or incompatible detergent ingredients may reduce enzyme activity. Practical formulation testing is recommended to determine optimal operating conditions.
Recommended Dosage and Usage Instructions
The recommended dosage is 0.2 to 1.0% w/w based on the total weight of the finished detergent formulation.
| Detergent Batch | 0.2% Dosage | 0.5% Dosage | 1.0% Dosage |
|---|---|---|---|
| 10 kg | 20 g | 50 g | 100 g |
| 50 kg | 100 g | 250 g | 500 g |
| 100 kg | 200 g | 500 g | 1 kg |
| 500 kg | 1 kg | 2.5 kg | 5 kg |
General Application Instructions
- Prepare the liquid detergent base according to the intended formulation.
- Confirm that the formulation pH is compatible with the enzyme blend.
- Allow the detergent mixture to reach a suitable temperature before enzyme addition.
- Add HIOL at the recommended dosage of 0.2 to 1.0% by weight.
- Mix gently until the enzyme blend is uniformly distributed.
- Evaluate enzyme stability and cleaning performance before commercial production.
Important: Actual dosage should be optimized through laboratory testing because detergent ingredients and processing conditions may influence enzyme performance.
Application Areas
Liquid Laundry Detergents
HIOL is suitable for liquid laundry detergent formulations designed to remove common household stains, including protein deposits, starch residues, and greasy contamination. The combination of three enzymes helps improve overall cleaning versatility.
Liquid Laundry Detergent Pods
HIOL can be evaluated as an enzymatic cleaning component in liquid-filled laundry pods. Compatibility with concentrated detergent ingredients and water-soluble pod films should be confirmed through formulation and stability testing.
Textile Washing Applications
The enzyme blend supports the removal of organic stains from compatible washable fabrics. It may be evaluated in household and commercial textile washing systems requiring multi-stain cleaning performance.
Concentrated Liquid Detergents
HIOL may be incorporated into compatible concentrated liquid detergent formulations. Enzyme stability should be evaluated carefully because high surfactant concentrations, preservatives, solvents, and other ingredients may affect activity during storage.
Formulation Compatibility
When incorporating HIOL into detergent formulations, compatibility with other ingredients should be evaluated to maintain enzyme stability and cleaning efficiency.
- Surfactants: Evaluate compatibility with the selected anionic, nonionic, and other surfactant systems.
- pH: Maintain suitable conditions for enzyme activity and stability.
- Oxidizing Agents: Strong oxidizing ingredients may reduce enzyme activity.
- Preservatives: Verify compatibility with preservatives and other formulation additives.
- Storage Stability: Test retained enzyme activity and physical stability during the intended product shelf life.
Storage Conditions
Store HIOL in a tightly sealed container at a recommended temperature of 2 to 25°C in a cool, dry environment.
Avoid direct sunlight, excessive heat, and unnecessary exposure to incompatible chemicals. Storage at lower temperatures within the recommended range can help preserve enzyme activity and extend the usable storage period.
Keep the product securely closed when not in use and avoid contamination during handling.
Safety and Handling
HIOL is a concentrated liquid enzyme preparation. Enzyme proteins may cause allergic reactions or sensitization in susceptible individuals.
- Avoid direct contact with skin and eyes.
- Avoid inhaling enzyme-containing aerosols or mists.
- Wear appropriate protective gloves and eye protection.
- Use adequate ventilation during industrial handling.
- If skin contact occurs, rinse thoroughly with clean water.
- Follow appropriate industrial hygiene and safety procedures.




