Food Grade Powder Neutral Protease Enzyme For Protein Hydrolysis
Neutral protease is produced by submerged fermentation of Bacillus subtilis followed by purification and formulation. It can catalyze the hydrolysis of protein to produce free amino acids and peptides at around neutral pH. Because of its fast hydrolysis under mild conditions and easy control, the product has been widely used in alcohol fermentation, leather softening and depilation, silk degumming, vegetable and animal protein hydrolysis, brewing, juice clarification, baking and feed, etc.
Introduction
Neutral protease is produced by submerged fermentation of Bacillus subtilis followed by purification and formulation. It can catalyze the hydrolysis of protein to produce free amino acids and peptides at around neutral pH. Because of its fast hydrolysis under mild conditions and easy control, the product has been widely used in alcohol fermentation, leather softening and depilation, silk degumming, vegetable and animal protein hydrolysis, brewing, juice clarification, baking and feed, etc.
Mechanism
Protease catalyzes the cleavage of peptide bonds in a polypeptide (protein) chain, producing smaller molecular peptides and amino acids. The endo-type of protease can only liberate peptides, while exo-type can remove amino acid at one of the terminals of a peptide chain to produce amino acid. The following diagram illustrates how a protease works on a peptide:
Product Standard
The product complies with GB1886.174.
| No. | Items | Index |
| 1 | Particle size (% <40 mesh) /(%) | ≥80 |
| 2 | Loss on drying/(%) | ≤8.0 |
| 3 | Lead/(mg/kg) | ≤5.0 |
| 4 | Arsenic/(mg/kg) | ≤3.0 |
| 5 | Total viable count/(CFU/g) | ≤50000 |
| 6 | Coliform Bacteria/(CFU/g) | ≤30 |
| 7 | Escherichia coli (CFU/g) | <10 |
| (MPN/g) ≤3.0 | ||
| 8 | Salmonella/(25g) | Not Detected |
Application
The successful applications include:
- Vegetable and animal protein hydrolysis for health food or other purposes
- Meat tenderization
- Wine clarification
- Soy sauce fermentation
Baking Industry
The following benefits can be seen when the Neutral Protease has been used:
- Dough handling property modification
- Biscuit color improvement
- Reduced use of pyrosulfite and additives
Brewing Industry
For brewing, the product helps increase FAN level in wort especially when specific adjunct like barley is used, and prevent cold haze of beer.
Recommended Dosage
The recommended dosage is 0.01-5 kg/t DS. The dosage has to be optimized based on each application, the raw material specifications, product expectation, and processing parameters. It is better to begin the test with the convenient volume.
Warnings
Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until it’s finished.
Safety
Enzyme preparations are proteins that may induce sensitization and cause allergic-type symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes, or nasal mucosa. Any direct contact with the human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.
Package And Storage
Package: 1 kg / bag.
Storage: Keep sealed in a dry and cool place and avoid direct sunlight.
Shelf life: 12 months in a dry and cool place.
