Bulk Papain Enzyme Supplier

Food-grade papain from Carica papaya for meat tenderization, protein hydrolysis, brewing, and nutraceuticals. 80,000–2,000,000 U/g. Ships to 180+ countries from 1 kg.
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How Papain Works

Papain (EC 3.4.22.2) is a cysteine protease extracted from unripe papaya latex (Carica papaya). It cleaves peptide bonds with broad substrate specificity, making it one of the most versatile plant proteases in industrial use.

Broad-Spectrum Proteolysis

Papain cleaves peptide bonds adjacent to basic and hydrophobic amino acid residues with minimal selectivity — making it highly effective for complete protein hydrolysis across diverse substrates including meat, plant proteins, and collagen.

Wide Temperature Range

Active from 20°C to 70°C with optimum at 60–65°C, and retains significant activity in meat at cooking temperatures (50–70°C). Self-inactivates above 75°C — enabling controlled tenderization through cook temperature.

Natural & Non-GMO

Papain is extracted directly from unripe papaya fruit by coagulation, drying, and purification of the natural latex. No genetic modification involved. Suitable for natural and clean-label food products.

Papain Applications by Industry

Papain is used wherever plant-derived proteolysis provides clean-label, cost-effective protein processing.
肉类嫩化

Injected or applied as marinade to beef, pork, poultry, and game. Degrades myofibrillar proteins and connective tissue during cooking, producing tender texture. Active until inactivation at 75°C+ for controlled results.

Protein Hydrolysates

Produces savory flavor extracts, yeast extract, plant protein hydrolysates, and collagen peptides. High-activity grades (1,000,000–2,000,000 U/g) enable cost-effective bulk hydrolysate production.

Brewing & Chill-Proofing

Liquid papain is applied during beer conditioning to hydrolyze haze-forming proteins, preventing chill-haze and improving beer clarity and filtration in lager and ale production.

Nutraceuticals

High-activity papain powder (400,000–2,000,000 U/g) is used in digestive enzyme supplements, protein digestion aids, and anti-inflammatory nutraceutical formulations. Food-grade and pharmaceutical-grade available.

Feed & Specialty Processing

Improves protein digestibility in aquaculture and specialty animal feeds. Also used in collagen peptide production, wound debridement preparations, and specialty fermentation processes.

Papain Applications: Process Details, Dosage & Parameters

肉类嫩化

Meat Processing | Injection Brine & Marinade Systems

功能： Papain hydrolyzes myofibrillar proteins (actin, myosin) and connective tissue collagen, breaking rigid muscle-fiber cross-links. Warner-Bratzler shear-force values improve 30–60% at commercial dosages. Activity is mild during cold storage and spikes on cooking, providing even, cook-activated tenderizing.

典型用量 10–100 ppm in injection brine (10–15% pump rate); 0.005–0.03% w/w dry rub; activity ≥500,000 USP units/g

应用： Beef and pork primals (blade, round, chuck), injection tenderizing lines, marinade systems, automated brine injection.

相关指南 Meat Processing Enzymes — bromelain, protease & tenderizing enzyme guide →

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Protein Hydrolysates

Food Ingredients | FPH & Savory Hydrolysate Production

功能： Broad-spectrum cysteine protease active at pH 5–8, up to 70°C. Rapidly cleaves peptide bonds generating oligopeptides and free amino acids. Degree of hydrolysis (DH) of 15–40% is achievable in 2–6 hours, producing savory flavor precursors, functional peptides, or nutritional protein supplements.

典型用量 0.1–0.5% w/w enzyme:substrate; DH target 15–40%; 50–60°C, pH 6–7, 2–6 hour reaction

应用： Meat protein hydrolysates, fish protein hydrolysates (FPH), soy hydrolysates, seasoning bases, infant formula protein.

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Brewing & Chill-Proofing

Brewing | Haze-Active Protein Removal

功能： Chill haze forms when haze-active hordein-derived proteins bind polyphenols at low temperatures. Papain degrades these proteins during conditioning without affecting foam stability (beta-glucan and foam-positive proteins are largely unaffected at correct dosage).

典型用量 1–5 g/hL beer; add to conditioning tank at 0–4°C; inactivated by pasteurization at 72°C

应用： Lager and pilsner chill-proofing, bottle-conditioned ale shelf-life extension, keg beer clarity.

相关指南 Brewing Enzymes — amylase, glucoamylase & haze-control enzyme guide →

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保健食品和补充剂

Digestive Enzymes | GRAS-Recognized Plant Protease

功能： At physiological pH 5–7, papain assists protein digestion in the stomach and small intestine. Standardized papain (6M–10M USP units/g) is used in digestive enzyme blends, sports nutrition recovery products, and anti-inflammatory supplement formulations.

典型用量 10,000–50,000 USP units per serving; typically 100–500 mg capsule/tablet; pH stability 4–8

应用： Digestive enzyme blends, sports recovery supplements, anti-inflammatory nutraceuticals, protein digestion aids.

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Animal Feed & Pet Food

Feed Manufacturing | Protein Digestibility Improvement

功能： Papain pre-digests feed proteins, increasing amino acid bioavailability for monogastric animals (poultry, swine, pets) with low intrinsic protease output. Improved digestibility reduces feed conversion ratio (FCR) and supports growth performance.

典型用量 100–500 U/g feed; 0.01–0.05% inclusion in pet food; heat-stable formulations available for pelleted feed

应用： Poultry and swine performance feed, premium pet food, soft-moist pet treats, pre-digested protein concentrates.

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Papain Products Available Now

Browse our papain range — from 80,000 to 2,000,000 U/g in powder form, plus liquid preparations. All products ship with certificate of analysis and technical data sheet.

Why Source Papain from enzymes.bio?

低起订量（1 公斤起

从研发样品到全面生产，订单数量灵活。准备就绪后即可扩大规模。.

定制制剂

我们的技术团队可根据您的具体要求定制酶制剂。.

全球航运

DHL、联邦快递和美国邮政（USPS）向 180 多个国家提供全程跟踪服务。提供冷链运输。.

技术支持

我们的酶专家就用量、稳定性和配方提供应用指导。.

常见问题

Papain is a natural cysteine protease extracted from the latex of unripe Carica papaya fruit. The latex is collected by scoring the green fruit, then processed by precipitation, filtration, and drying to produce crude or refined papain powder. Our commercial papain is available in activity grades from 500,000 to 10,000,000 USP units/g.
Grade selection depends on application: food-grade tenderizing typically uses 500K–2M USP/g; brewing and nutraceuticals prefer 3M–6M USP/g purity grades; feed and industrial applications may use crude grades. Contact us with your target DH or tenderizing specification.
Minimum order is 1 kg for sample/trial orders. Standard commercial orders are 25 kg drums. We ship globally from warehouse stock with typical lead times of 3–7 business days for stocked grades.
Yes — papain is derived from Carica papaya (plant-based) and is inherently halal. Kosher documentation is available upon request. We supply CoA, halal certificate, and kosher letter for each batch.
Papain from natural papaya latex is generally permitted as a processing aid in organic food production. It is listed as permitted under EU organic regulations and recognized as natural by the USDA NOP. We provide audit documentation upon request.
For injection tenderizing: 10–50 ppm in brine at 10–15% pump rate. For dry rub: 0.005–0.03% w/w. Higher dosages cause over-tenderizing (mushy texture). For marinade systems: 20–100 ppm in an acidic carrier (pH 4–6) to stabilize during cold storage.
Both are cysteine proteases but differ in specificity. Papain has broader activity — cleaves both myofibrillar proteins and collagen — and remains active to 85°C. Bromelain (from pineapple) is more specific for myofibrillar proteins with lower collagen activity. For tough, collagen-rich cuts (shank, shoulder), papain is preferred.
Papain has reduced but measurable activity at 4–10°C, which is why injection-tenderized meats continue softening during chill storage. Optimal activity is 50–70°C. Papain is fully inactivated by cooking (>72°C), so no enzymatic activity remains in cooked product.
Yes — papain is effective for plant protein hydrolysis: soy, pea, wheat gluten, and rice proteins. It generates savory umami peptides and reduces anti-nutritional factors. Papain hydrolysis at pH 6–7 produces highly soluble peptides suitable for plant-based food and beverage formulations.
Store in sealed containers at 4–15°C, away from moisture and direct sunlight. At room temperature (25°C), activity loss is approximately 5–10%/month. Refrigerated storage maintains >90% activity for 12–18 months. Avoid freeze-thaw cycles for liquid preparations.

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