Food Grade Liquid Protease Enzyme To Avoid Protein Cold Turbidity
Introduction
Protease (endoprotease) is produced by submerged fermentation of Aspergillus niger followed by purification and formulation. It’s a highly specific protease that only cleaves peptide bonds formed by proline at C-terminals. The unique property makes it very beneficial in brewing and beverage to prevent cold haze and improve non-biological stability.
Mechanism
Cold haze in beer and beverages is formed mainly due to the interactions between haze sensitive proteins and polyphenols, where prolines in peptides are involved. Proline specific protease can modify the sensitivity by attacking the peptide bonds formed by the amino acids specifically, and prevent the formation of haze. Due to its high specificity, the enzyme will bring no harm to other parts of the protein, keeping all other functionalities of the protein.
Product Standard
The product complies with GB1886.174.
| No. | Items | Index | |
| 1 | Bulk density/(g/ml) | 1.05-1.20 | |
| 2 | pH/(25℃) | 3.5–5.5 | |
| 3 | Lead/(mg/kg) | ≤5.0 | |
| 4 | Arsenic/(mg/kg) | ≤3.0 | |
| 5 | Total viable count/(CFU/mL) | ≤50000 | |
| 6 | Coliform Bacteria/(CFU/mL) | ≤30 | |
| 7 | Escherichia coli | (CFU/mL) | <10 |
| (MPN/mL) | ≤3.0 | ||
| 8 | Salmonella/(25mL) | Not Detected |
Application
Beer colloidal stability improvement: The enzyme can be used to substitute PVPP in the prevention of cold haze in beer without harming the foaming property of beer and health concerns.
Juice and beverage clarification: Likewise, the enzyme can also be applied to solve the cold haze problem and extend the shelf life stability of juice or beverage products by damaging the haze sensitivity of proline-containing proteins.
Recommended Dosage
The recommended dosage is 0.01-0.5kg/t DS. The dosage has to be optimized based on each application, the raw material specifications, product expectation, and processing parameters. It is better to begin the test with a convenient volume.
Warnings
Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until it’s finished.
Safety
Enzyme preparations are proteins that may induce sensitization and cause allergic types of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes, or nasal mucosa. Any direct contact with the human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.
Package and Storage
Package: 1 kg / bag.
Storage: Keep sealed in a dry and cool place and avoid direct sunlight.
Shelf life: 12 months in a dry and cool place.
