Neutral Protease Enzyme for Alcohol Industry Enzymes.Bio Prn50
Introduction
The neutral protease is produced by submerged fermentation of Bacillus subtilis followed by purification and formulation. The product has been widely used in alcohol fermentation to degrade proteins in raw materials.
Definition Of Unit
1 unit of Neutral Protease equals the amount of enzyme, which hydrolyzes casein to get 1μg of tyrosine at 30℃ and pH7.5 in 1 min.
Characteristics
| Declared Activity | 50000 u/g |
| Production Organism | Bacillus subtilis |
| Physical Form | Powder |
| Color | Snuff color, color can vary from batch to batch. Color intensity is not an indication of enzyme activity. |
| Odour | Normal microbial fermentation odour. |
Specifications
| No. | Items | Index | |
| 1 | Particle size (%<40 mesh) | ≥80 | |
| 2 | Loss on drying/(%) | ≤8.0 | |
| 3 | Lead/(mg/kg) | ≤5.0 | |
| 4 | Arsenic/(mg/kg) | ≤3.0 | |
| 5 | Total viable count/(CFU/g) | ≤50000 | |
| 6 | Coliform Bacteria/(CFU/g) | ≤30 | |
| 7 | Escherichia coli | (CFU/g) | <10 |
| (MPN/g) | ≤3.0 | ||
| 8 | Salmonella/(25g) | Not Detected |
Recommended Dosage
The recommended dosage is 0.05-3 kg of the enzyme preparation per ton of total raw materials. However, the optimal dosage depends on the application criteria, the processing parameters being utilized, and the attributes desired in the hydrolysate being developed.
Package
Packaging specification: 1 kg / bag.
Storage
When stored as recommended, the product is best used within 12 months from the date of delivery.
Storage at customer’s warehouse: 0-15℃.
This product should be stored in a cool and dry place in a sealed container, avoiding insolation, high temperature, and damp. The product has been formulated for optimal stability. Extended storage or adverse conditions such as higher temperature or higher humidity may lead to a higher dosage requirement.
Safety
Enzyme preparations are proteins, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes, or nasal mucosa, so any direct contact with the human body should be avoided. If irritation or allergic response for skin or eyes develops, consult a doctor.
