How Xylanase Works
Cuts Xylan Chains
Endo-xylanase cleaves internal xylosidic bonds, rapidly reducing arabinoxylan viscosity and releasing soluble oligomers for downstream processing or fermentation.
Redistributes Dough Water
In wheat flour, xylanase solubilizes water-binding arabinoxylans — transferring bound water to gluten and starch, improving dough extensibility and oven spring.
Breaks Down Cell Walls
In feed and biofuel, xylanase degrades plant cell wall polysaccharides, releasing nutrients and fermentable sugars otherwise locked inside hemicellulose.
Xylanase Applications by Industry
ベーキング
Increases loaf volume by 10–15%, improves crumb texture, and extends shelf life. Used in bread, rolls, tortillas, and other wheat-based products.
飼料
Reduces digesta viscosity in poultry and swine diets, improving feed conversion ratio and energy digestibility of wheat, rye, and barley-based feeds.
パルプ・紙
Pre-bleaching kraft pulp with xylanase reduces chlorine chemical demand by up to 20%, cutting costs while improving pulp brightness and environmental profile.
Biofuel
Degrades hemicellulose in lignocellulosic biomass to release fermentable pentose sugars, improving ethanol yield in second-generation bioethanol production.
醸造・飲料
Reduces wort viscosity in wheat and rye beers, improving lautering speed and filtration. Also used in juice clarification and fruit wine production.
Xylanase Applications: Process Details, Dosage & Parameters
製パン・製粉
Baking | Arabinoxylan Modification & Dough Improvement
関数： Xylanase hydrolyzes arabinoxylans (pentosans) in wheat flour, releasing water-binding insoluble arabinoxylans and improving dough extensibility, water redistribution, and gluten network formation. This reduces mixing time, improves machinability, and produces a more open crumb structure — especially valuable for whole wheat and high-fiber formulations.
典型的な投与量： 0.5–5 ppm based on flour weight; add directly to flour or at mixing stage; pH 5–6 in dough
用途: Pan bread, whole wheat bread, baguette, high-fiber bread, industrial bread production, frozen dough.
関連ガイド Baking Enzymes — xylanase, amylase & dough improver dosage guide →
飼料
Feed Manufacturing | Anti-Nutritional Factor (ANF) Reduction
関数： Arabinoxylans in wheat, rye, and barley-based diets increase intestinal viscosity, reducing nutrient absorption and worsening wet litter in poultry. Exogenous xylanase hydrolyzes these viscosity-causing arabinoxylans, improving nutrient digestibility and energy utilization. This reduces feed cost and improves growth performance.
典型的な投与量： 500–2,000 U/g feed; thermostable grades required for pelleting (≥85°C); combine with beta-glucanase for maximum effect in barley diets
用途: Wheat-based broiler and layer feeds, rye and barley diets, swine wheat-bran diets, aquafeed with plant proteins.
Pulp & Paper (Bleach Boosting)
Paper Manufacturing | Chlorine-Free Bleaching
関数： Xylanase removes xylan redeposited on cellulose fibers during kraft pulp cooking, which blocks bleaching chemical penetration. Enzymatic xylan removal (bio-bleaching pre-treatment) reduces chlorine dioxide demand in ECF bleaching by 15–25% and improves final pulp brightness. This is the most commercially significant industrial xylanase application.
典型的な投与量： 0.05–0.2% w/w on pulp; 50–60°C; pH 7–9 (alkaline-stable xylanase required); 1–2 hours pre-bleach treatment
用途: Kraft pulp ECF bleaching, TCF bleaching sequences, paper brightness improvement, bleach chemical cost reduction.
Biofuel & Biorefinery
Lignocellulosic Biomass | Hemicellulose Saccharification
関数： Xylan (hemicellulose) constitutes 15–30% of agricultural biomass and is the second most abundant polysaccharide after cellulose. Xylanase hydrolyzes xylan to xylose, which can be fermented to ethanol by pentose-fermenting yeasts or converted to xylitol. Xylanase also improves cellulose accessibility in biomass by removing hemicellulose sheathing.
典型的な投与量： 0.05–0.2% w/w on dry biomass; 50–55°C; pH 5–6; 24–48 hours; combine with cellulase for maximum biomass conversion
用途: 2G bioethanol from agricultural residues, xylose production, xylitol manufacturing, biorefinery co-product valorization.
Brewing & Beverages
Brewing | Wort Viscosity & Filtration Improvement
関数： Arabinoxylans contribute to wort viscosity and filtration resistance, particularly in wheat beer and barley-adjunct brewing. Xylanase hydrolysis reduces wort viscosity and improves lautering and filtration efficiency. Combined with beta-glucanase, xylanase is standard in adjunct brewing enzyme cocktails for high-viscosity grain bills.
典型的な投与量： 0.01–0.05% w/w; add during mashing; 55–65°C; 30–60 minutes
用途: Wheat beer, barley adjunct brewing, rye beer, oat stout processing, adjunct mashing optimization.
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