Bulk Papain Enzyme Supplier

Food-grade papain from Carica papaya for meat tenderization, protein hydrolysis, brewing, and food manufacturing. 80,000–2,000,000 U/g. Ships to 180+ countries from 1 kg.
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How Papain Works

Papain (EC 3.4.22.2) is a cysteine protease extracted from unripe papaya latex (Carica papaya). It cleaves peptide bonds with broad substrate specificity, making it one of the most versatile plant proteases in industrial use.

Broad-Spectrum Proteolysis

Papain cleaves peptide bonds adjacent to basic and hydrophobic amino acid residues with minimal selectivity — making it highly effective for complete protein hydrolysis across diverse substrates including meat, plant proteins, and collagen.

Wide Temperature Range

Active from 20°C to 70°C with optimum at 60–65°C, and retains significant activity in meat at cooking temperatures (50–70°C). Self-inactivates above 75°C — enabling controlled tenderization through cook temperature.

Natural & Non-GMO

Papain is extracted directly from unripe papaya fruit by coagulation, drying, and purification of the natural latex. No genetic modification involved. Suitable for natural and clean-label food products.

Papain Applications by Industry

Papain is used wherever plant-derived proteolysis provides clean-label, cost-effective protein processing.
Τρυφεροποίηση κρέατος

Injected or applied as marinade to beef, pork, poultry, and game. Degrades myofibrillar proteins and connective tissue during cooking, producing tender texture. Active until inactivation at 75°C+ for controlled results.

Protein Hydrolysates

Produces savory flavor extracts, yeast extract, plant protein hydrolysates, and collagen peptides. High-activity grades (1,000,000–2,000,000 U/g) enable cost-effective bulk hydrolysate production.

Brewing & Chill-Proofing

Liquid papain is applied during beer conditioning to hydrolyze haze-forming proteins, preventing chill-haze and improving beer clarity and filtration in lager and ale production.

Παραγωγή τροφίμων

High-activity papain powder (400,000–2,000,000 U/g) is used in food processing, meat tenderization, and cosmetic formulations. Food-grade and industrial-grade available.

Feed & Specialty Processing

Improves protein digestibility in aquaculture and specialty animal feeds. Also used in collagen peptide production, wound debridement preparations, and specialty fermentation processes.

Papain Applications: Process Details, Dosage & Parameters

Τρυφεροποίηση κρέατος

Meat Processing | Injection Brine & Marinade Systems

Λειτουργία: Papain hydrolyzes myofibrillar proteins (actin, myosin) and connective tissue collagen, breaking rigid muscle-fiber cross-links. Warner-Bratzler shear-force values improve 30–60% at commercial dosages. Activity is mild during cold storage and spikes on cooking, providing even, cook-activated tenderizing.

Τυπική δοσολογία: 10–100 ppm in injection brine (10–15% pump rate); 0.005–0.03% w/w dry rub; activity ≥500,000 USP units/g

Εφαρμογές: Beef and pork primals (blade, round, chuck), injection tenderizing lines, marinade systems, automated brine injection.

Σχετικός οδηγός: Meat Processing Enzymes — bromelain, protease & tenderizing enzyme guide →

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Protein Hydrolysates

Food Ingredients | FPH & Savory Hydrolysate Production

Λειτουργία: Broad-spectrum cysteine protease active at pH 5–8, up to 70°C. Rapidly cleaves peptide bonds generating oligopeptides and free amino acids. Degree of hydrolysis (DH) of 15–40% is achievable in 2–6 hours, producing savory flavor precursors, functional peptides, or functional food ingredients.

Τυπική δοσολογία: 0.1–0.5% w/w enzyme:substrate; DH target 15–40%; 50–60°C, pH 6–7, 2–6 hour reaction

Εφαρμογές: Meat protein hydrolysates, fish protein hydrolysates (FPH), soy hydrolysates, seasoning bases, infant formula protein.

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Brewing & Chill-Proofing

Brewing | Haze-Active Protein Removal

Λειτουργία: Chill haze forms when haze-active hordein-derived proteins bind polyphenols at low temperatures. Papain degrades these proteins during conditioning without affecting foam stability (beta-glucan and foam-positive proteins are largely unaffected at correct dosage).

Τυπική δοσολογία: 1–5 g/hL beer; add to conditioning tank at 0–4°C; inactivated by pasteurization at 72°C

Εφαρμογές: Lager and pilsner chill-proofing, bottle-conditioned ale shelf-life extension, keg beer clarity.

Σχετικός οδηγός: Brewing Enzymes — amylase, glucoamylase & haze-control enzyme guide →

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Food Manufacturing Applications

Food Processing Enzyme | GRAS-Recognized Plant Protease

Λειτουργία: Papain is active across pH 5.0–8.0, making it suitable for diverse industrial hydrolysis applications. Standardized papain (6M–10M USP units/g) is used in food processing, protein hydrolysis, and commercial enzyme formulations.

Τυπική δοσολογία: applied at 0.1–0.5% w/w in food processing; pH stability 4–8

Εφαρμογές: Food processing enzymes, protein hydrolysis, cosmetic formulations, commercial enzyme blends.

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Animal Feed & Pet Food

Feed Manufacturing | Protein Digestibility Improvement

Λειτουργία: Papain pre-digests feed proteins, increasing amino acid bioavailability for monogastric animals (poultry, swine, pets) with low intrinsic protease output. Improved digestibility reduces feed conversion ratio (FCR) and supports growth performance.

Τυπική δοσολογία: 100–500 U/g feed; 0.01–0.05% inclusion in pet food; heat-stable formulations available for pelleted feed

Εφαρμογές: Poultry and swine performance feed, premium pet food, soft-moist pet treats, pre-digested protein concentrates.

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Papain Products Available Now

Browse our papain range — from 80,000 to 2,000,000 U/g in powder form, plus liquid preparations. All products ship with certificate of analysis and technical data sheet.
Top picks: Papain 1.5 Million U/g High Activity

Why Source Papain from enzymes.bio?

Χαμηλό MOQ από 1 kg

Ευέλικτες ποσότητες παραγγελίας από δείγματα Ε&Α έως πλήρη παραγωγή. Κλιμάκωση όταν είστε έτοιμοι.

Προσαρμοσμένες προετοιμασίες

Η τεχνική μας ομάδα διαμορφώνει προσαρμοσμένα παρασκευάσματα ενζύμων που ανταποκρίνονται στις ακριβείς προδιαγραφές σας.

Παγκόσμια ναυτιλία

DHL, FedEx και USPS σε 180+ χώρες με πλήρη παρακολούθηση. Διαθέσιμη αποστολή με ψυχρή αλυσίδα.

Τεχνική υποστήριξη

Καθοδήγηση εφαρμογής από τους ειδικούς μας στα ένζυμα σχετικά με τη δοσολογία, τη σταθερότητα και τη σύνθεση.

Συχνές ερωτήσεις

Η παπαΐνη είναι μια φυσική πρωτεάση κυστεΐνης που εξάγεται από το λατέξ του άγουρου καρπού Carica papaya. Το λατέξ συλλέγεται με χαράξεις στον πράσινο καρπό και στη συνέχεια επεξεργάζεται με καθίζηση, διήθηση και ξήρανση για την παραγωγή ακατέργαστης ή ραφιναρισμένης σκόνης παπαΐνης. Η εμπορική μας παπαΐνη διατίθεται σε βαθμίδες δραστικότητας από 500,000 έως 10,000,000 μονάδες USP/g.
Grade selection depends on application: food-grade tenderizing typically uses 500K–2M USP/g; brewing and food manufacturing prefer 3M–6M USP/g purity grades; feed and industrial applications may use crude grades. Contact us with your target DH or tenderizing specification.
Minimum order is 1 kg for sample/trial orders. Standard commercial orders are 25 kg drums. We ship globally from warehouse stock with typical lead times of 3–7 business days for stocked grades.
Yes — papain is derived from Carica papaya (plant-based) and is inherently halal. Kosher documentation is available upon request. We supply CoA, halal certificate, and kosher letter for each batch.
Papain from natural papaya latex is generally permitted as a processing aid in organic food production. It is listed as permitted under EU organic regulations and recognized as natural by the USDA NOP. We provide audit documentation upon request.
For injection tenderizing: 10–50 ppm in brine at 10–15% pump rate. For dry rub: 0.005–0.03% w/w. Higher dosages cause over-tenderizing (mushy texture). For marinade systems: 20–100 ppm in an acidic carrier (pH 4–6) to stabilize during cold storage.
Both are cysteine proteases but differ in specificity. Papain has broader activity — cleaves both myofibrillar proteins and collagen — and remains active to 85°C. Bromelain (from pineapple) is more specific for myofibrillar proteins with lower collagen activity. For tough, collagen-rich cuts (shank, shoulder), papain is preferred.
Papain has reduced but measurable activity at 4–10°C, which is why injection-tenderized meats continue softening during chill storage. Optimal activity is 50–70°C. Papain is fully inactivated by cooking (>72°C), so no enzymatic activity remains in cooked product.
Yes — papain is effective for plant protein hydrolysis: soy, pea, wheat gluten, and rice proteins. It generates savory umami peptides and reduces anti-nutritional factors. Papain hydrolysis at pH 6–7 produces highly soluble peptides suitable for plant-based food and beverage formulations.
Store in sealed containers at 4–15°C, away from moisture and direct sunlight. At room temperature (25°C), activity loss is approximately 5–10%/month. Refrigerated storage maintains >90% activity for 12–18 months. Avoid freeze-thaw cycles for liquid preparations.

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