Product Description
This product is a 5′-Phosphodiesterase preparation produced by submerged fermentation of Penicillium citrinum followed by purification, formulation, and drying. Nuclease has important applications in industrial production of 5′-nucleotide and savory product production like yeast extract.
Function and Application
- Industrial production of 5′-nucleotides.
- Industrial production of high nucleotides extract.
Mechanism
Nucleases act on (3 ‘, 5 ‘) -phosphodiester bonds of single-stranded DNA or RNA to form 5 ‘-nucleotides. The enzyme also breaks down the 3 ‘-phosphate monoester bond in a nucleotide or oligonucleotide.
Product Standard
| ITEMS | INDEX | |
| Particle size (%<40 mesh) | ≥80 | |
| Loss on drying/(%) | ≤8.0 | |
| Lead/(mg/kg) | ≤5.0 | |
| Arsenic/(mg/kg) | ≤3.0 | |
| Total viable count/(CFU/g) | ≤50000 | |
| Coliform Bacteria/(CFU/g) | ≤30 | |
| Escherichia coli (CFU/g) | <10 | |
| (MPN/g) | ≤3.0 | |
| Salmonella/(25g) | Not Detected |
Reaction Parameters
| CONDITION | RANGE |
| Activity Temperature | 45℃-85℃ |
| Optimum Temperature | 65℃-75℃ |
| Activity pH | 4.0-8.0 |
| Optimum pH | 4.5-5.5 |
Recommended Dosage
The recommended dosage is 2-5%/t. The dosage has to be optimized based on each application, the raw material specifications, product expectation, and processing parameters. It is better to begin the test with the convenient volume.
Safe Handling Precautions
Enzyme preparations are proteins that may induce sensitization and cause allergic-type symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes, or nasal mucosa. Any direct contact with the human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.
Package
Storage: Keep sealed in a dry and cool place and avoid direct sunlight.
Shelf Life
Shelf life: 12 months in a dry and cool place.
